不同种黄草石斛的HPLC指纹图谱研究  

Study on HPLC fingerprint in different species of Dendrobii Caulis

作　　者：郝普彦[1] 郭力[1] 许莉[1] 石媛慧[1] 陈佳江[1] 韦练[1] 罗方利[1] 张廷模[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075

出　　处：《中药与临床》2013年第2期1-3,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：科技部"十二五"支撑项目--石斛规范化种植基地及其SOP优化;升级研究(2011BAI13B02-8)

摘　　要：目的:采用HPLC-DAD-ELSD联用法对五种黄草石斛的指纹图谱进行对比研究。方法:采用DikmaC18(250mm×4.6mm,5μm)色谱柱;乙腈-0.5%冰乙酸水溶液梯度洗脱;柱温:30℃;流速:1mL.min-1;DAD串联蒸发光散射检测器检测。结果:建立了五种黄草石斛的HPLC指纹图谱,并标定了8个指纹峰。相似度结果表明,各种石斛相似度较低,差别较大。结论:建立的指纹图谱方法稳定、可靠、重现性好,可直接了解石斛品种与化学成分变化的关系。Objective: To set up the fingerprint chromatograms of chemical principles of five kinds of Dendrobii Caulis by HPLC-DAD-ELSD. Method: Analysis was performed at 30℃ on a Dikma Cl8 column(4.6 mm×250 mm,5 μm) with a mobile phase consisting of acetonitrile-0.5% glacial acetic acid, gradient eluted at the flow rate of 1.0 mL·min-1 with DAD and ELSD detection. Result: HPLC fingerprint of Dendrobii Caulis was established, and 8 peaks were detected based on it. Similarity among different species was low. Conclusion: The method is stable, reliable and repeatable. It’s useful for the study on the relationship between chemical components and species of Dendrobii Cauli.

关 键 词：黄草石斛 指纹图谱 HPLC-DAD-ELSD联用 

分 类 号：R917[医药卫生—药物分析学]