黄药子配伍甘草分煎液与合煎液中黄独乙素含量的比较  

Comparison of Diosbulbin B in different decoctions of Dioscorea Dulbifera Rhizome and Glycyrrhiza Uralensis

作　　者：华碧春[1] 马丽娜[2] 史道华[3] 陈小峰[4] 

机构地区：[1]福建中医药大学药学院,福建福州350003 [2]成都中医药大学,四川成都611137 [3]福建省妇幼保健院,福建福州350004 [4]福建省第二人民医院,福建福州350004

出　　处：《中药与临床》2013年第2期50-53,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金课题(项目批准号:81274105)

摘　　要：目的:比较黄药子配伍甘草前后毒性成分黄独乙素含量的变化。方法:采用PLATISILTM-C18(4.6mm×250mm,5μm)色谱柱,以乙腈-0.1%冰乙酸(40:60)为流动相,检测波长为210nm,柱温为25℃。结果:黄独乙素在37.5μg.mL-1～310.0μg.mL-1呈良好的线性,r=0.9999。黄独乙素含量:黄药子单煎液>分煎液>合煎液。结论:黄药子配伍甘草后其毒性成分黄独乙素含量有所下降,可能是其配伍减毒的原因之一。Objective:To compare the contents of Diosbulbin B in the separated and mixed decoction of Dioscorea bulbifera rhizome and Glycyrrhiza uralensis.Method:The samples were separated at 25 ℃ on a PLATISILTM-C18 column eluted with the mobile phase of acetonitrile-water-glacialacetic acid(40:60:0.1).Flow rate was 0.8 mL﹒min-1 and the detection wavelength was set at 210 nm.Result:The calibration curve was linear within the range from 37.5 μg﹒mL-1 to 310 μg﹒mL-1(r=0.9999).The content of Diosbulbin B in Dioscorea bulbifera rhizome was higher than separated decoction,and which was higher than mixed decoction.Conclusion:The decrease of Diosbulbin B content may be account for the toxicity attenuation after compatibility.

关 键 词：黄药子 甘草 黄独乙素 HPLC 含量测定 

分 类 号：R917[医药卫生—药物分析学]