A Quantitative Method for Simultaneous Determination of Four Anthraquinones with One Marker in Rhei Radix et Rhizoma  

作　　者：Jing-jing ZHU Zhi-min WANG Xin-yu MA Wei-hong FENG Qi-wei ZHANG 

机构地区：Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China[1] Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China[2]

出　　处：《Chinese Herbal Medicines》2012年第2期157-163,共7页中草药（英文版）

基　　金：National Youth Fund (30901961)

摘　　要：Objective To develop a quantitative method for simultaneously determining multi-components in Rhei Radix et Rhizoma using one chemical reference substance.Methods The contents of multi-components were calculated by the UV relative correction factors(RCFs)of chrysophanol,physcion,and rhein to emodin.Results The values of RCFs at 274 nm for rhein,chrysophanol,and physcion to emodin were 0.712,0.674,and 1.051.The calibration curves were linear over the ranges of 0.02-4.08,0.02-4.12,0.07-12.92,and 0.02-3.68μg/mL for rhein,emodin, chrysophanol,and physcion,respectively.The contents of emodin in 18 samples were determined by the external standard method,and the contents of the other three anthraquinone aglycones were calculated according to their RCFs. Conclusion No significant difference is found in comparison with the classical method,indicating that the RCFs have high reliability within their linear ranges and could be used in quality control of Rhei Radix et Rhizoma.The quantitative analysis of multi-component with a single marker is especially suitable for herbal medicines containing unstable or hard to be purified components as quality control markers.Objective To develop a quantitative method for simultaneously determining multi-components in Rhei Radix et Rhizoma using one chemical reference substance.Methods The contents of multi-components were calculated by the UV relative correction factors(RCFs)of chrysophanol,physcion,and rhein to emodin.Results The values of RCFs at 274 nm for rhein,chrysophanol,and physcion to emodin were 0.712,0.674,and 1.051.The calibration curves were linear over the ranges of 0.02-4.08,0.02-4.12,0.07-12.92,and 0.02-3.68μg/mL for rhein,emodin, chrysophanol,and physcion,respectively.The contents of emodin in 18 samples were determined by the external standard method,and the contents of the other three anthraquinone aglycones were calculated according to their RCFs. Conclusion No significant difference is found in comparison with the classical method,indicating that the RCFs have high reliability within their linear ranges and could be used in quality control of Rhei Radix et Rhizoma.The quantitative analysis of multi-component with a single marker is especially suitable for herbal medicines containing unstable or hard to be purified components as quality control markers.

关 键 词：ANTHRAQUINONE multi-component quantitative analysis quality control Rhei Radix et Rhizoma UV relative correction factor 

分 类 号：R284[医药卫生—中药学]