Simultaneous Determination of Six Quaternary Ammonium Alkaloids in Coptidis Rhizoma by UPLC  

作　　者：QIU Ling-ling CHEN Long-hu YAN Dan ZHANG Ping TAN Man-rong DU Xiao-xi XIAO Xiao-he 

机构地区：[1]China Military Institute of Chinese Materia Medica,Integrative Medicine Centre,302 Military Hospital,Beijing 100039,China [2]College of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China [3]College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611139,China [4]Centre for Drug Reevaluation,State Food and Drug Administration,Beijing 100045,China

出　　处：《Chinese Herbal Medicines》2012年第2期164-169,共6页中草药（英文版）

基　　金：National Natural Science Foundation of China(30873385,81173542);the Beijing Natural Science Foundation(7112128);the Open Research Fund of State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicinal Resources

摘　　要：Objective To establish a new,rapid,and reliable reversed-phase ultra performance liquid chromatography (RP-UPLC)method for the simultaneous determination of six quaternary ammonium alkaloids(QAAs)in Coptidis Rhizoma.Methods The effect of different experimental parameters on the analysis of QAAs by RP-UPLC was evaluated.Results Optimal resolution was achieved with an Acquity UPLC BEH C18 column using a gradient elution profile and a mobile phase consisting of water spiked with 10 mmol/L ammonium bicarbonate(A,pH adjusted to 10.0 by ammonia water)and acetonitrile(B),at a flow rate of 0.30 mL/min and wavelength of 345 nm. The column temperature was set at 30℃.The proposed method was found to be reproducible,precise,and rapid according to the method validation.Conclusion The proposed method,which is compatible with MS analysis and the preparation of QAA,provides some helpful insights into the quality control of Coptidis Rhizoma.Objective To establish a new,rapid,and reliable reversed-phase ultra performance liquid chromatography (RP-UPLC)method for the simultaneous determination of six quaternary ammonium alkaloids(QAAs)in Coptidis Rhizoma.Methods The effect of different experimental parameters on the analysis of QAAs by RP-UPLC was evaluated.Results Optimal resolution was achieved with an Acquity UPLC BEH C18 column using a gradient elution profile and a mobile phase consisting of water spiked with 10 mmol/L ammonium bicarbonate(A,pH adjusted to 10.0 by ammonia water)and acetonitrile(B),at a flow rate of 0.30 mL/min and wavelength of 345 nm. The column temperature was set at 30℃.The proposed method was found to be reproducible,precise,and rapid according to the method validation.Conclusion The proposed method,which is compatible with MS analysis and the preparation of QAA,provides some helpful insights into the quality control of Coptidis Rhizoma.

关 键 词：Coptidis Rhizoma method validation quaternary ammonium alkaloids simultaneous determination ultra performance liquid chromatography 

分 类 号：TQ461[化学工程—制药化工]