Transformation of Compound K from Saponins in Leaves of Panax notoginseng by Immobilized β-Glucanase  

作　　者：DONG Hui-juan1,2,JIANG Bin-hui3,HAN Ying3,GENG Yong1,ZHAO Yu-qing4 1.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China 2.Graduate School of Chinese Academy of Sciences,Beijing 100039,China 3.School of Resources and Civil Engineering,Northeastern University,Shenyang 110004,China 4.Shenyang Pharmaceutical University,Shenyang 110016,China 

出　　处：《Chinese Herbal Medicines》2010年第1期-,共7页中草药（英文版）

基　　金：ZHAO Yu-qing was supportive with a Liaoning Modernization TCM grant (LN403004),China

摘　　要：Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Alg 2),were evaluated for their ability to immobilize β-glucanase.The amount and purity of C-K obtained from the transformation process were analyzed by HPLC,and the immobilizing parameters were optimized.Results β-Glucanase can be immobilized and reused with either of the entrapment.However,using Alg 1 resulted in higher enzyme activity than Alg 2.The optimal concentration of the immobilized enzyme was 10%;The optimal crosslinking time was 4–6 h;and the optimal concentration of the crosslinking agent was 6%– 7%.Conclusion Immobilized β-glucanase shows sustained enzyme activity,good ethanol tolerance,and was reusable for the preparation of C-K from SLPN.Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Alg 2),were evaluated for their ability to immobilize β-glucanase.The amount and purity of C-K obtained from the transformation process were analyzed by HPLC,and the immobilizing parameters were optimized.Results β-Glucanase can be immobilized and reused with either of the entrapment.However,using Alg 1 resulted in higher enzyme activity than Alg 2.The optimal concentration of the immobilized enzyme was 10%;The optimal crosslinking time was 4–6 h;and the optimal concentration of the crosslinking agent was 6%– 7%.Conclusion Immobilized β-glucanase shows sustained enzyme activity,good ethanol tolerance,and was reusable for the preparation of C-K from SLPN.

关 键 词：Β-GLUCANASE ginsenoside compound K IMMOBILIZATION saponins in leaves of Panax notoginseng(Burk.) F.H.Chen transformation 

分 类 号：S567.239[农业科学—中草药栽培]