Isolation and Purification of Isoaloeresin D and Aloin from Aloe vera by High-speed Counter-current Chromatography  

作　　者：WAN Jin-zhi1,CHEN Xin-xia1,QIU Chun-mei1,LIN Xiang-cheng2 1.Laboratory for Pharmaceutical Analysis and Quality Evaluation,School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China 2.Institute of Fine Chemicals and Synthetic Drugs,Sun Yat-sen University,Guangzhou 510006,China 

出　　处：《Chinese Herbal Medicines》2010年第2期148-152,共5页中草药（英文版）

基　　金：Science and Technology Project of Zhuhai (PC20051072),2005

摘　　要：Objective To develop an efficient method to isolate and purify the main components isoaloeresin D and aloin from Aloe vera for its industrial production.Methods High-speed counter-current chromatography was used to isolate isoaloeresin D and aloin in a one-step separation from dried crude extract of A.vera.The biphasic solvent system composed of hexane-ethyl acetate-acetone-water(0.2:5:1.5:5) was used at a flow rate of 1.0 mL/min,while the lipophilic phase was selected as the mobile phase and the apparatus was rotated at 840 r/min.The effluent was detected at 254 nm.Results Isoaloeresin D(53.1 mg) and aloin(106.9 mg) were separated from the crude extract(384.7 mg) with the purities of 98.6% and 99.5%,respectively.Conclusion HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.Objective To develop an efficient method to isolate and purify the main components isoaloeresin D and aloin from Aloe vera for its industrial production.Methods High-speed counter-current chromatography was used to isolate isoaloeresin D and aloin in a one-step separation from dried crude extract of A.vera.The biphasic solvent system composed of hexane-ethyl acetate-acetone-water(0.2:5:1.5:5) was used at a flow rate of 1.0 mL/min,while the lipophilic phase was selected as the mobile phase and the apparatus was rotated at 840 r/min.The effluent was detected at 254 nm.Results Isoaloeresin D(53.1 mg) and aloin(106.9 mg) were separated from the crude extract(384.7 mg) with the purities of 98.6% and 99.5%,respectively.Conclusion HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.

关 键 词：Aloe vera ALOIN high-speed counter-current chromatography isoaloeresin D extraction and purification 

分 类 号：Q946[生物学—植物学]