人趋化因子诱骗受体D6基因真核表达载体的构建及鉴定  

Construction and identification of eukaryotic expression vector of chemokine decoy receptor D6 gene

作　　者：李强[1] 赵良中[1] 陈爽[1] 李文彬[1] 李妍[1] 

机构地区：[1]吉林医药学院病原生物学教研室,吉林吉林132013

出　　处：《吉林医药学院学报》2013年第1期1-3,共3页Journal of Jilin Medical University

基　　金：吉林医药学院青年科研基金(院资[2009-07]号)

摘　　要：目的构建并鉴定人趋化因子诱骗受体D6基因的真核表达载体。方法 PCR扩增目的基因片段,与载体分别双酶切后连接,产物转化入JM109感受态细菌,筛选阳性克隆酶切鉴定并测序。结果构建的pCDNA3.1-hD6-his真核表达载体序列正确。结论成功构建了人趋化因子诱骗受体D6的真核表达载体。Objective To construct and identify the eukaryotic expression vector of chemokine decoy receptor D6 gene.Methods D6 gene was amplified by PCR,followed by double-enzyme cleavage and connection with vector.The production then was transformed into JM109 cells,subsequently the positive colony were selected and identification by enzyme cleavage before sequencing.Results The sequence of constructed eukaryotic expression vector pCDNA3.1-hD6-his was correct.Conclusion The eukaryotic expression vector pCDNA3.1-hD6-his has been constructed successfully.

关 键 词：D6 趋化因子受体 诱骗受体 真核表达载体 

分 类 号：R392[医药卫生—免疫学]