MEM培养基对带状疱疹减毒活疫苗卡那霉素残留量的影响  

作　　者：李生军 刘彬婷 皮景学 彭岩松 高明珠 梁雪 李海燕 梁慧颖 隋波 闫磊 赵海波 赫宝双 

机构地区：[1]长春祈健生物制品有限公司,吉林长春130012

出　　处：《生物技术世界》2013年第4期51-51,53,共2页Biotech World

摘　　要：目的:分析比较MEM培养基对带状疱疹减毒活疫苗卡那霉素残留量的影响。方法:分别使用海克隆、日水2种MEM培养基培养2BS细胞,经传代、感染、洗涤、收获、合并、冻干等工序制备带状疱疹减毒活疫苗,分析比较MEM培养基对带状疱疹减毒疫苗卡那霉素残留量的影响。结果:日水MEM培养基制备的带状疱疹减毒活疫苗卡那霉素残留量一般是海克隆MEM培养基制备疫苗的5-10倍。结论:美国海克隆实验公司的MEM培养基卡那霉素残留量明显小于日水MEM培养基。Objective :To compare the effects of two kinds of MEM medium on residual Kanamycin in herpes zoster vaccine.Methods :The human embryo lung diploid cells(2BS) were cultrued in Hyclone or Nissui MEM medium.We preparation of herpes zoster vaccines though a series of processes include of cell cultrue,virus infection,harvested,freeze-dried.We compare the effects of two MEM medium on residual Kanamycin in herpes zoster vaccine.Results :The residual Kanamycin in vaccine by Nissui MEM medium is 5-10 fold more than the Hyclone MEM medium.Conclusion :The residual Kanamycin in vaccine by Hyclone MEM medium is loss than the Nissui MEM medium.

关 键 词：MEM 人胚肺二倍体细胞 卡那霉素残留量 

分 类 号：R392[医药卫生—免疫学]