近红外透射法的葡萄糖水溶液的浓度测量和分析  

Measurement and Analysis of Glucose Solution with Near Infrared Transmission Spectroscopy

作　　者：郭川军[1] 

机构地区：[1]哈尔滨金融学院计算机系,哈尔滨150030

出　　处：《生物医学工程研究》2013年第3期153-157,共5页Journal Of Biomedical Engineering Research

基　　金：国家自然科学基金资助项目(61201017);黑龙江省科技攻关项目(GC12C108);中央高校基本科研业务费专项资金资助项目(HIT.NSRIF.2013010)

摘　　要：葡萄糖浓度测量是无创检测人体血糖浓度的基础研究,具有重要的科学意义。本研究利用近红外光谱技术(NIRS)和回归定标方法相结合测定葡萄糖水溶液浓度。实验采用傅里叶光谱仪对葡萄糖溶液的近红外透射光谱进行采集,利用光谱数据建立回归模型,讨论偏最小二乘法(PLS)校正模型中主因子数的确定方法,并对不同预处理方法对PLS模型建立影响进行了研究。采用Savitzky-Golay卷积平滑对溶液样品吸光度光谱进行预处理,通过交叉验证方法求取预测残差平方和(PRESS),葡萄糖溶液浓度预测值的最大相对误差限制在了8%以内。研究结果表明,近红外透射法用于葡萄糖水溶液浓度测量能够达到一定的测试精度,具有进一步的研究价值。The measurement of glucose concentration is the basis for the noninvasive detection of blood glucose concentration, which is significant in scientific research.In the present study,Near Infrared Spectrum(NIRS)and regression analysis were combined to measure the glucose concentration. The spectrum of glucose solutions was obtained with the Fourier Transformed Infrared Spectrometer,and the data was used for further regression analysis.In this study,the methodology of determining partial least squares (PLS)components and spectral preprocessing were discussed.During PLS modeling,using Savitzky -Golay and the Prediction Residual Error Sum of Squares (PRESS),we achieved the maximum relative error within 8%.The experiment results demonstrat that NIRS has the potential to the measurement of glucose solution and noninvasive detecting of blood glucose.

关 键 词：近红外光谱 偏最小二乘 卷积平滑 交叉验证 预测残差平方和 

分 类 号：R318[医药卫生—生物医学工程]