叠鞘石斛多糖含量测定两种方法的比较  

The method comparison of determining polysaccharide content in Dendrobium denneanum Kerr

作　　者：吴强[1] 夏厚林[1] 张廷模[1] 郭帅[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2013年第5期4-5,11,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：十二五科技支撑计划项目"石斛规范化种植SOP优化升级研究"(No.2011BAI1BO2-8)

摘　　要：目的:通过叠鞘石斛多糖的含量研究,比较改良苯酚硫酸法和传统苯酚硫酸法测定多糖的差异。方法:分别进行两种方法的准确度、重复性、精密度、稳定性等试验及测定6批次叠鞘石斛多糖含量。结果:改良苯酚硫酸法测定叠鞘石斛中多糖含量优于传统苯酚硫酸法。结论:改良苯酚硫酸法操作简便、稳定可靠,可用于叠鞘石斛多糖含量的检测。Objective:To select a more accurate and convenient method of detecting Dendrobium denneanum Kerr.polysaccharide content. Method: The accuracy, repeatability, precision, stability were tested for the two methods, and polysaccharide contents in six batches Dendrobium denneanum Kerr. were detected. Result: The comparison showed that the better method to detect the content was improved phenol sulfuric colorimetric acid method. Conclusion: Improved phenol-sulfuric acid method is more stable and accurate than traditional phenol sulfuric acid colorimetric method, and it can be be used to detect polysaccharide content in Dendrobium denneanum Kerr.

关 键 词：叠鞘石斛 多糖 改良苯酚硫酸法 传统苯酚硫酸法 

分 类 号：R284[医药卫生—中药学]