超声波提取-恒波长同步荧光法测定淡水藻中叶绿素a的含量  

Determination of Chlorophyll a in Green Algae by Ultra sound Extraction and Synchronous Fluorescence Spectrometry

作　　者：吴俊森[1] 万修志[1] 马永山[1] 孙韶华[2] 

机构地区：[1]山东建筑大学市政与环境工程学院,山东济南250101 [2]济南市供排水监测中心,山东济南250033

出　　处：《中国人口·资源与环境》2011年第S1期192-194,共3页China Population,Resources and Environment

基　　金：国家水体污染控制与治理科技重大专项"引黄供水地区水质风险评估技术研究与应用"(编号:2008ZX07422-001)

摘　　要：针对绿藻,采用超声波提取,以荧光激发-发射波长差216nm,建立了同步荧光法测定绿藻叶绿素a含量的新方法,其线性范围为0.02～1.25 mg/L,检出限为1.6ug/L,加标回收率在97.0%～104%之间。与分光光度法测定叶绿素a的对比试验结果表明,两者无显著性差异,但该方法具有快速、灵敏,其他常见色素不干扰测定的优点,能够满足供水中绿藻叶绿素a含量快速检测的需要。A method for the determination of chlorophyll a in chlorella by ultra-sound extraction and synchronous fluorescence spectrometry with constant—wavelength interval of 216 nm is developed.The linear range is 0.02-1.25 mg/L with the detection limit of 1.6ug/L.The recovery is between 97.0% and 104%.By comparison with the visible spectrophotography,they have not significant differences.The method is quick and sensitive.Other common pigments do not interfere.It can meet the demands of testing green algae.

关 键 词：同步荧光 超声波提取 叶绿素A 绿藻 

分 类 号：X832[环境科学与工程—环境工程] O657.3[理学—分析化学]