过氧化物模拟酶β- 环糊精- 氯化血红素催化氧化苯酚测定过氧化氢(英)  

Phenol as Hydrogen Donor for Determination of Hydrogperoxide Catalyzed by β-CD-hemin as Mimetic Enzyme of Peroxidase

作　　者：黄应平[1] 袁丁[2] 李瑞萍[1] 

机构地区：[1]湖北三峡学院理工学院化学系 [2]湖北三峡学院医学院基础部,宜昌443000

出　　处：《三峡大学学报（人文社会科学版）》1999年第5期43-48,共6页Journal of China Three Gorges University(Humanities & Social Sciences)

摘　　要：基于Ｈ２Ｏ２ ＋４ － 氨基胺替吡啉＋ 苯酚———醌式染料（λｍａｘ５０７ｎｍ） ＋ Ｈ２Ｏ 反应，研究了不同过氧化物酶测定Ｈ２Ｏ２ 的方法－ 在ｐＨ７－０２ Ｔｒｉｓ－ ＨＣｌ 缓冲溶液中，系统研究了辣根过氧化物酶（ＨＲＰ） 及模拟酶的催化活性，其中β－ 环糊精－ 氯化血红素催化Ｈ２Ｏ２ 氧化苯酚与４ － 氨基胺替吡啉生成醌式染料反应的催化活性最高，研究了β－ 环糊精－ 氯化血红素作为模拟酶的显色反应条件和在显色反应中β－ 环糊精－ 氯化血红素和底物的最佳浓度０～６－８×１０－ ５ｍｏｌ·Ｌ－ １ 过氧化氢浓度范围测定具有好的重线性和精确度，表观摩尔吸光系数为１－１０ ×１０４Ｌ·ｍｏｌ－ １·ｃｍ － １－Various mimetic enzymes of peroxidase have been used a catalyst insted of peroxidase in determination of hydrogen peroxide based on reaction: H 2O 2+4-aminoantipyrine+phenol→quinon dye (λ max 507nm)+H 2O In the pH7 02 Tris-HCl buffer solution, the difference of relative catalytic activity was systematic studied between natural enzyme (HRP) and mimetic enzymes β-CD-hemin was the best one of the substitutions for peroxidase in determination of hydrogen peroxide by formation of dye from phenol and 4-aminoantipyrine The optimal concentration of mimetic enzyme and substrate were studied in the β-CD-hemin-Phenol-H 2O 2 system Meanwhile the optimal condition of color reaction was investigated 0～6 8×10 -5 mol·L -1 concentration rage of hydrogen peroxide was determinated with good accuracy and reproducibility The apparent molar absorptivity for hydrogen peroxide was 1 10×10 4L·mol -1 ·cm -1

关 键 词：酶催化反应 β-环糊精-氯化血红素 苯酚 4-氨基胺替吡啉 

分 类 号：O629[理学—有机化学]