大鼠第Ⅲ脑室接触脑脊液生长抑素mRNA阳性细胞的观察  

Observation of Somatostatin m-RNA Positive and CSF Contacting Cells in the Wall of Third Ventricle in Rat

作　　者：张建国[1] 付承英[1] 王尊琼[1] 汪红[1] 黎家华[1] 

机构地区：[1]湖北三峡学院医学院医学基础部,宜昌443000

出　　处：《三峡大学学报（人文社会科学版）》1999年第2期64-66,共3页Journal of China Three Gorges University(Humanities & Social Sciences)

摘　　要：采用地高辛标记的反意生长抑素cRNA探针原位杂交技术 ,研究接触脑脊液生长抑素mRNA阳性细胞在第Ⅲ脑室壁的分布 显示生长抑素mRNA阳性细胞分布于第Ⅲ脑室、从视前区 (数目为 73 8± 8 7)到正中隆起区 (2 7± 1 3 3)的室管膜 ,以室旁核区数目最多 (40 5 5±2 9 6)To study the distribution of somatostatin(SOM) mRNA-positive neurons in the wall of the third ventricle in rats In situ hybridization with antisense SOM mRNA probe-labled digoxigenin was applied SOM mRNA-positives cells were found distributed in parts of the wall of the third ventricle from preoptic region (73 8±8 7) to region of median eminence (27 4±13 2), But mainly in the region of the pararentricular nucleas (405 5±29 6) The findings have supplied molecular biological evidence on the physiological roles of somatostin in the central nervous system

关 键 词：生长抑素 第Ⅲ脑室 原位杂交 大鼠 

分 类 号：Q95-3[生物学—动物学]