健肾颗粒剂对糖尿病肾病大鼠肾脏组织TGF-β1、ET-1mRNA及肾功能的影响  

Effect of Jianshen Granule on Kidney TGF-β1,ET-1mRNA and Function of Rats with Diabetic Nephrosis(DN)

作　　者：徐丽梅[1] 马建伟[1] 马新英[1] 魏汉林[1] 董静[1] 张文龙[1] 蔡庆[1] 

机构地区：[1]解放军空军总医院,北京100036

出　　处：《浙江中医药大学学报》2010年第3期307-309,共3页Journal of Zhejiang Chinese Medical University

摘　　要：[目的]观察中药健肾颗粒剂对DN大鼠肾脏组织中TGF-β1、ET-1mRNA及肾功能影响。[方法]采用5/6肾切除加尾静脉注射STZ的方法复制大鼠DN动物模型,并随机分成健肾颗粒剂、苯拉普利、保肾康、病理对照及正常对照组,采用生化法检测肾功能,PCR方法检测肾脏组织中TGF-β1、ET-1mRNA,组织病理学方法观察肾脏组织结构。[结果]健肾颗粒剂组大鼠血中Cr、BUN水平低于苯拉普利、保肾康及病理对照组(P<0.05或P<0.01);健肾颗粒剂组肾脏组织中TGF-β1、ET-1mRNA表达明显低于其他三组(P<0.05或P<0.01),肾脏组织病理结构也较其他三组有明显改善;健肾颗粒剂降低DN大鼠血糖、尿蛋白及血脂的作用明显优于苯拉普利、保肾康及病理对照组(P<0.05或P<0.01)。[结论]健肾颗粒剂可明显改善DN大鼠肾功能,并有降血糖、调整血脂,下调DN大鼠肾脏TGF-β1、ET-1mRNA的作用。[Objective]To explore the effects of TCM Jianshen Granule on kidney TGF-β1,ET-1mRNA and function of rats.[Method]Take 5/6 kidney resection and tail vein injection of STZ to copy rats DN model,and randomly divide them into groups of Jianshen Granule(1)Benazeprilat(2),Baoshen Kang(3),pathological control(4)and normal control(5),take bio-chemistry method to observe kidney tissue.[Result]The TGF-β1 and ET-1 mRNA expression in group 1 is markedly lower than other 3 groups,so is its kidney pathological structure much relieved;Jianshen Granule is much better than Benazeprilat,Baoshen Kang and group 4 on reducing glucose,urinary protein and lipid.[Conclusion]Jianshen Granule can obviously improve DN rats’ kidney function,also reduce glucose,regulate lipid,down-regulate TGF-β1 and ET-1mRNA as well.

关 键 词：糖尿病肾病 健肾颗粒剂 转化生长因子-Β1 内皮素-1 

分 类 号：R285.5[医药卫生—中药学]