土壤中昆虫病原线虫种群密度检测新方法  

New Method for Population Density Detection of EPN in Soil

作　　者：战丽莉[1,2] 王义 许艳丽[1] 裴希超 刘振宇[5] 

机构地区：[1]中国科学院东北地理与农业生态研究所,中国科学院,黑土区农业生态院重点实验室,海伦农田生态系统国家野外观测研究站 [2]黑河学院物理化学系 [3]Rutgers University,New Brunswick NJ 08901-8520,USA [4]黑河出入境检验检疫局 [5]黑龙江省农业科学院植物脱毒苗木研究所

出　　处：《土壤与作物》2013年第2期71-76,共6页Soils and Crops

基　　金：宁夏农业综合开发项目(NTKJ2012-05-01)

摘　　要：采用室内模拟试验,对昆虫病原线虫检测器收集到昆虫病原线虫线虫数量和土壤中昆虫病原线虫密度相关性进行研究,确定昆虫病原线虫检测器收集时间、检测器放置间距进行了试验研究。结果表明,收集时间和检测器放置间距对检测器收集到线虫数量有显著影响,48h为最佳收集时间,检测器之间放置8cm为最佳间距。检测器中收集到昆虫病原线虫的数和昆虫病原土壤中线虫密度显著相关,相关系数R2达到0.9364。该技术为昆虫病原线虫定性及定量检测提供了一种新方法。laboratory simulation test was used for this research,the relation between population density of EPN in soil and the individual number in detection tube was studied. Collection time and the distance between detection tubes were studied by one way test. The results showed that collection time and the distance between detection tubes affected the individual number in detection tube significantly. 48 hours was the best Collection time and 8cm was the best distance between detection tubes for the EPN collection. The result showed that the individual number in detection tube was related to the population density of EPN in soil ,the highest R2 was 0. 9364. This research provide a new method for qualitative and quantitative of EPN detection.

关 键 词：昆虫病原线虫 种群密度 检测 新方法 

分 类 号：S476+.9[农业科学—农业昆虫与害虫防治]