阿片肽对背根节神经元电活动的影响及八肽胆囊收缩素和血管紧张素Ⅱ的抗阿片作用  

Effects of Opioids on Electrical Activity of Dorsal Root Ganglion Neurons and Anti-opoid actions of Cholecystokinin octapeptide and Angiotensin Ⅱ

作　　者：王过渡[1] 李重庆 韩济生[1] 赵志奇[2] 

机构地区：[1]北京医科大学神经科学中心,北京100083 [2]中国科学院上海脑研究所,上海200031

出　　处：《三峡大学学报（人文社会科学版）》1998年第3期96-104,共9页Journal of China Three Gorges University(Humanities & Social Sciences)

摘　　要：应用细胞内记录方法分别观察μ、δ和κ阿片受体激动剂DAMGO、DPDPE及U50488H对成年大鼠离体背根节神经元（dorsalrootganglion，DrG）动作电位和膜电位的作用．结果显示：DAMCO和U50488H能缩短DRG神经元动作电位的Ca2+成分电位的时程．在静息膜电位水平，DAMGO和DPDPE（100nmol～1μmol/L）能引起大部分DRG神经元（DAMGO：42．7％，DPDPE：36．45％）产生超极化和小部分神经元（DAMGO：7．29％，DPDPE：11．45％）产生去极化反应，这些反应能分别被μ-和δ-阿片受体的选择性桔抗剂β-FNA及Naltrindole阻断；同浓度的U50488H对DRG神经元膜电位没有明显影响．八肽胆囊收缩素（cholecystokininoctapetide，CCK－8）能完全翻转DAMGO的超极化作用．CCK－8的翻转作用能被CCKB受体桔抗剂L365260取消．血管紧张素Ⅱ（angiotensinⅡ，AⅡ）翻转有些DRG神经元的DPDPE的超极化作用，而对一些神经元的DPDPE介导的超极化反应无效．AⅡ受体的选择性粘抗剂saralasin阻断AⅡ的翻转作用．结果揭示：μ-阿片受体能介导抑制动作电位中的Ca2+电流和降低细胞膜的兴奋性；δ-阿片受体主要介导对膜兴奋性的抑制；κ-阿片受体仅介导抑制动作电位中的Ca2+电流，CCK和AⅡ的抗阿片作用是通过细胞内机制起作用，而不是通过直接的通?The effects of μ-,δ-and κ-.opioid receptor anonists,DAMGO,DPDPE and resting rnembrane potentials of adult rat dorsal root ganglion neurons.The results showed that 100 nmol/L～1μmol/L DAMGO and DPDPE could induce hyperpolarization responses of 42.7% and 36.45% neurons,and they also produce depolarization reactions of 7.329% and 11.45% neurons,aspectively.The effects could be separately blocked by μ-and δ-opioid receptor antagonists,β -FNA and Natrindole.There were not the effects with concentration as same as DAMGO and DPDPE of U50488H on the neurons.100nmol/L～1μmol/L DAMGO amd U50488H could decrease action potential duration(APD)of some neurons,which might be blocked by their selective antago nists β-FNA and Nor-BNI. Although 1～5μM cholecystokkinin octapeptide(CCK-8)and angiotensin Ⅱ(AⅡ)had not evoked any response on the neuronal resiting membrane potential(RP)and action potential(AP),bu CCK-8 could reverse the responese to DANGO on the neuronal RP and AP.it is of interest to note that AⅡ could in turn be abolished L365260,an antagonist of CCKB receptor,or sanalasin,an antagonist of AⅡ receptor. The results suggest that μ-opoid receptor mediated the inhibition on either Ca2+ inward cur rent iu action potential and cellular exeitativity,δ-opioid receptor mainly on cellular excitativity and κ-opioid meceptor only on the Ca2+ inward current in adult rat DRG neurons.The anti-opioid effects of CCK-8 and All receptor with opioid receptors could coexist in some DRG neurons.

关 键 词：阿片受体 八肽胆囊收缩素 血管紧张素Ⅱ 膜电位 背根节神经元 成年大鼠 

分 类 号：R333[医药卫生—人体生理学]