H9N2亚型禽流感病毒人工感染蛋鸡的组织病理学研究  

Studies on the histopathology of H9N2 subtype influenza virus experimentally inoculated into layers

作　　者：罗玲[1] 胡薛英[1] 程国富谷长勤 周全[1] 李自力[1] 赵雅心[1] 周诗其[1] 毕丁仁[1] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070

出　　处：《中国预防兽医学报》2004年第4期259-261,265,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：本研究用H9N2亚型禽流感病毒人工感染蛋鸡 ,对感染蛋鸡的组织病理学进行了观察 ,结果发现接毒后第 3d ,主要病理变化为全身各组织的广泛性充血、出血以及肝脏、肾脏的变性、坏死 ;肝、肾组织的Mann亚甲蓝伊红染色发现肝细胞、肾小管上皮细胞胞浆内有嗜酸性包涵体 ;红细胞凝集抑制试验 (HI试验 )检查结果表明鸡接毒 3d后 ,HI抗体开始呈上升趋势。H9N2 subtype Influenza virus was inoculated intranasally into forty four-week old layers.At 3 days post inoculation,gross histopathological changes showed broad hyperemia,hemorrhage and degeneration ,necrosis in liver and kidney.Eosinophilic inclusion bodies were found in the cytoplasm of hepatocytes and the renal tubular epithelial cells by Mann staining methods for liver and kidney.Antiserum against H9N2 subtype AIV was tested with the hemagglutinin inhibition(HI),the findings indicated that HI antibody rised at 3 days post inoculation.

关 键 词：禽流感病毒 H9N2亚型 组织病理学 包涵体 蛋鸡 

分 类 号：S858.31[农业科学—临床兽医学]