RT-PCR技术检测猪瘟病毒的应用研究  

An applied studies on diagnosis of hog cholera by reverse transcriptase polymerase chain reaction

作　　者：罗廷荣[1] 莫扬[1] 吴文德[2] 黄玉华[1] 黄伟坚[1] 秦爱珍[1] 刘芳[1] 温荣辉[1] 陆芹章[1] 余克伦[1] 

机构地区：[1]广西大学动物科技学院预防兽医学实验室,广西南宁530005 [2]广西大学动物疫病防治研究所,广西南宁530005

出　　处：《中国预防兽医学报》2004年第4期307-309,312,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：广西大学博士科研启动基金 (X9830 3) ;广西区教育厅资助项目 (T9810 6 )

摘　　要：本试验应用反转录_聚合酶链式反应 (RT_PCR)对猪瘟进行诊断应用研究。应用RT_PCR对来自广西不同地区的 135份疑似猪瘟病料进行检测 ,84份诊断为阳性 ,阳性率 6 2 2 %。从百色、柳州地区等采集的健康猪扁桃体和淋巴结共 2 76份 ,经RT_PCR检测 ,37份为阳性 ,阳性率为 13 4 %。其中健康猪扁桃体带毒较高 ,2 4 6份扁桃体中有 35份阳性 ,占 14 2 %。采自柳州健康猪的 2 6份淋巴结材料全为阴性 ,只有邕宁县的 1份健猪淋巴结阳性。结果表明 ,RT_PCR技术可应用于猪瘟的临床诊断。In this study reverse transcriptase polymerase chain reaction (RT-PCR) was applied to diagnose hog cholera (HC).The result showed that 84 of 135 clinical samples from different places in Guangxi were positive, the rate of positive was 62.2 %.The samples of tonsils and lymph nodes that were collected from healthy pigs from Baise and Liuzhou have been detected.Among 276 tonsil samples of healthy pigs, 37 samples were positive, the rate of positive was 13.4 %.But only one lymph node sample of healthy pig from Yongning indicated positive.In which the samples of tonsil from healthy pigs showed higher rate of positive,35 of 246 tonsil samples were positive. The rate of positive was 14.2%.According to the results,RT-PCR is an useful method that can be applieded to diagnose HC clinically.

关 键 词：猪瘟 反转录-聚合酶链式反应 诊断 

分 类 号：S85253[农业科学—基础兽医学] S852.651[农业科学—兽医学]