非离子表面活性剂Tween-20胶束体系流体室温燐光法测定色氨酸  

Fluid room temperature phosphorescence of tryptophan in non-ionic surfactant Tween-20 micellar system

作　　者：龙文清[1] 江湘梅[1] 魏红[1] 

机构地区：[1]江西井冈山师范学院化学系,吉安343009

出　　处：《分析试验室》2004年第7期34-36,共3页Chinese Journal of Analysis Laboratory

基　　金：江西省教育厅资助项目 (赣教计字 [2 0 0 1]387号 )

摘　　要：提出了一种以非离子表面活性剂Tween 2 0为胶束介质、I-作重原子微扰剂、Na2 SO3 为除氧剂的胶束增稳室温光测定色氨酸的方法。详细研究了各种实验条件的影响。线性范围 8.0× 1 0 -8～ 8.0× 1 0 -6mol L ,检出限达 1 .4×1 0 -9mol L。直接用于大米、花生、大豆及竹笋中色氨酸的测定 ,相对标准偏差2 4%～ 3.3% ,与荧光法比较 ,相对误差 - 3.1 %～ 4.2 %。A method for determination of tryptophan (TRP) by micellar-stabilized room temperature phosphorescence (MS-RTP) with non-ionic surfactant Tween-20 as a micellar medium, I^- as a heavy atom perturber and sodium sulfite as a oxygen scavenger was described. The effect of various experimental conditions was studied in detail. The working curve of tryptophan had a linear range of 8.0×10^(-8)～8.0×10^(-6) mol/L with a detection limit of 1.4×10^(-9) mol/L. The method was applied to determination of tryptophan in rice, peanut, soybean and bamboo shoot samples with satisfactory results. The relative deviations were -3.1%～4.2% as compared with fluorimetry, and the relative standard deviations were 2.4%～3.3%.

关 键 词：非离子表面活性剂 胶束增稳室温燐光 色氨酸 碘化钾 

分 类 号：O657.3[理学—分析化学]