耐甲氧西林凝固酶阴性葡萄球菌中多种抗生素耐药基因的观察  

Observation of Multiple Antibiotic Resistant Genes in Methicillin-Resistant Coagulase Negative Staphylococci

作　　者：丁明东[1] 

机构地区：[1]泰州市人民医院感染科,江苏泰州225300

出　　处：《氨基酸和生物资源》2013年第2期51-55,共5页Amino Acids & Biotic Resources

摘　　要：探讨耐甲氧西林凝固酶阴性葡萄球菌(methicillin-resistant coagulase negative staphylococci,MRCNS)中多种抗生素耐药基因的分布情况,采取Kirby Bauer标准纸片扩散法(K-B纸片扩散法)对MRCNS开展药敏实验,并通过PCR检测菌种携带的抗生素耐药基因。药敏实验结果显示,96株MRCNS对庆大霉素、红霉素、莫匹罗星以及四环素的耐药性各有差异,但对氨苄西林的耐药性却达到100%,未发现对万古霉素具有耐药性的菌株;PCR检测结果显示,9种耐药基因merA、ermB、ermC、msrA、tetM、tetK、aac(6’)-aph(2")、aph(3’)-III、mupA的阳性检出率各有差异,没有扩增出vanA、ermA、vanB以及ant(6’)-I基因,但都扩增出mecA基因。结果表明,MRCNS可以同时携带多种抗生素耐药基因,属于一种多重耐药菌。To investigate the distribution of multiple antibiotic resistant genes in methicillin-resistant coagulase negative staphylococci(MRCNS),drug sensitivity test for MRCNS was done with K-B paper diffusion method,and antibiotic resistant genes in MRCNS were detected by PCR.Results of drug sensitivity test showed that the resistances of 96strains of MRCNS to gentamicin,erythromycin,mupirocin and tetracycline were different from each other,but all of 96strains were resistant to ampicillin while no vancomycin resistant strains were found.Results of PCR showed that the detection rates of 9antibiotic resistant genes,merA,ermB,ermC,msrA,tetM,tetK,aac(6’)-aph(2'),aph(3’)–III and mupA,were different;mecAgene was detected in all MRCNS while vanA,ermA,vanBor ant(6’)-I genes were not detected.The results showed that MRCNS belonged to a kind of multiple drug-resistant bacteria,and could carry multiple antibiotic resistant genes at the same time.

关 键 词：耐甲氧西林凝固酶阴性葡萄球菌 K-B纸片扩散法 耐药基因 耐药性 

分 类 号：Q5[生物学—生物化学]