牙鲆有丝分裂雌核发育二倍体的遗传特性分析  

The genetic characteristics of mitotic gynogenesis diploid in Japanese flounder Paralichthys olivaceus

作　　者：许丹丹[1,2] 刘永新[3] 王桂兴[2] 刘奕[4] 王玉芬[2] 刘海金[3,2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院北戴河中心实验站,河北秦皇岛066100 [3]中国水产科学研究院,北京100039 [4]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《大连海洋大学学报》2013年第6期542-548,共7页Journal of Dalian Ocean University

基　　金：国家鲆鲽类产业技术体系专项(CARS-50-G2);国家公益性农业行业专项(200903046)

摘　　要：对牙鲆Paralichthys olivaceus有丝分裂雌核发育后代进行了遗传差异分析,并将其与同一母本的减数分裂雌核发育二倍体和普通二倍体进行了比较。结果表明:有丝分裂雌核发育二倍体可分为两部分,即纯合子(Mitotic-A)和杂合子(Mitotic-B);Mitotic-A、Mitotic-B、减数分裂雌核发育二倍体(MGD)和牙鲆普通二倍体(ND)的平均纯合度分别为1.000 0、0.328 4、0.252 1、0.183 3,4个群体的亲代与子代之间的平均遗传相似系数分别为0.707 1、0.874 8、0.894 0、0.660 2,子代个体间的平均遗传相似系数分别为0.546 0、0.792 1、0.836 6、0.675 9,这表明Mitotic-A与Mitotic-B具有完全不同的遗传特征;MitoticB在poli110TUF、poli1906TUF位点纯合个体所占的比例较高(34.10%、73.86%),而MGD在这两个位点全部表现为杂合(0、0),Mitotic-B在poli1490TUF、poli1498TUF位点纯合个体所占的比例较小(10.23%、9.66%),而MGD在这两个位点全部表现为纯合(100%、100%),这说明诱导方式不同,重组交换的结果则不同。研究表明,Mitotic-B与MGD虽然都是杂合子,由于诱导方式不同,导致其遗传特征也不同。The genetic characteristics were compared between meiosis gynogenetic diploid( MGD) and normal diploid( ND) of Japanese flounder Paralichthys olivaceus using microsatellite marker. The results showed that the offsprings of mitotic gynogenesis were divided into homozygote( Mitotic-A) diploids and heterozygote( Mitotic-B) diploids. There was average homozygosity of 1. 000 0 in Mitotic-A,0. 328 4 in Mitotic-B,0. 252 1 in MGD and 0. 183 3 in ND. The genetic similarity between the parents and progenies were found to be 0. 707 1 in Mitotic-A, 0. 874 8 in Mitotic-B,0. 894 0 in MGD and 0. 660 2 in ND. The genetic similarity among progenies was shown to be 0. 546 0 in Mitotic-A,0. 792 1 in Mitotic-B,0. 836 6 in MGD,and 0. 675 9 in ND,indicating that there are significant differences in genetic characteristics between the Mitotic-A and Mitotic-B. The proportion of homozygous individuals was 34. 10% in Mitotic-B and 73. 86% at the poli110TUF and poli1906TUF loci,but 0 in MGD and 0 performance for heterozygosis completely. The homozygous individuals were found to represent 10. 23% in Mitotic-B and 9. 66% in the poli1490TUF and poli1498TUF loci,but to account for 100% in MGD and 100% performance for homozygosis completely. The findings show that there are different induction and information exchange approaches in Mitotic-B and MGD.

关 键 词：牙鲆 有丝分裂雌核发育 减数分裂雌核发育 微卫星 杂合子 

分 类 号：S917.4[农业科学—水产科学]