高灵敏度化学发光免疫法检测人血清中促甲状腺激素  

A Highly Sensitive Chemiluminescence Immunoassay Technique for Assaying TSH in Human Serum

作　　者：齐素文[1] 张会生[1] 刘昕宇[1] 李乔亮[1] 

机构地区：[1]深圳大学医学院生物医学工程系,广东深圳518060

出　　处：《发光学报》2013年第8期1089-1095,共7页Chinese Journal of Luminescence

摘　　要：建立了一种高灵敏度检测促甲状腺激素(TSH)的化学发光体系。将异鲁米诺(ABEI)和异硫氰酸荧光素(FITC)分别标记TSH的单克隆抗体,与待测TSH通过夹心法进行免疫反应形成抗原抗体复合物之后,加入抗-FITC抗体包被的磁珠,在磁场作用下加入缓冲液进行清洗,分离非特异性物质,加入发光底物NaOH和H2O2,检测发光强度(RLU)。本方法的灵敏度为0.01μIU/mL,批内和批间变异系数均在10%之内,回收率在96%～103%之间。使用本方法与其他检测系统进行比对,相关系数为0.993 7。实验结果表明,基于ABEI发光检测TSH的化学方法灵敏度高、结果可靠,具有较高的应用价值。A highly sensitive magnetic particle-based chemiluminescence immunoassay(CLIA) technique for assaying thyroid stimulating hormone(TSH) in serum was described.Fluorescein isothiocyanate(FITC) and N-(aminobutyl)-N-(ethylisoluminol)(ABEI) were used to label two different monoclonal antibodies of anti-TSH.Both of the labeled antibodies combined with TSH to form a sandwiched immunoreaction that was monitored by chemiluminescence(CL) detection.The magnetic particles(MPs) that were coated with anti-FITC antibody served as both the solid phase and the separator.The method showed high sensitivty with a detection limit of 0.01 μIU / mL.The coefficient of variation of intra-assay imprecision and the inter-assay imprecision were less than 10%.The average recoveries were 96% ～ 103%.A correlation analysis against an established automated assay generated a correlation coefficient of 0.993 7.The proposed method demonstrates an acceptable performance for quantifying serum TSH and is suitable for the clinical lab.

关 键 词：促甲状腺激素 异鲁米诺 化学发光免疫分析 

分 类 号：O482.31[理学—固体物理]