HPLC法测定双藤痹痛凝胶膏剂中雷公藤甲素和青藤碱的含量  

Determination of Triptolide and Sinomenine in Shuangteng Bitong Cataplasm by HPLC

作　　者：严国鸿[1] 褚克丹[2] 徐伟[2] 李煌[2] 夏裕发[2] 方宇骅 陈立典[3] 

机构地区：[1]福建中医药大学附属人民医院,福建福州350004 [2]福建中医药大学药学院,福建福州350122 [3]福建中医药大学,福建福州350122

出　　处：《福建中医药大学学报》2013年第5期27-30,共4页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省科技厅重大专项(2009YZ0001-1-1);十二.五国家科技支撑计划项目(2011BAI01B05)

摘　　要：目的控制双藤痹痛凝胶膏剂的质量。方法用HPLC法测定双藤痹痛凝胶膏剂中雷公藤甲素和青藤碱的含量。结果雷公藤甲素的线性范围为1.5216~15.2160μg/mL,平均回收率为98.07%;青藤碱的线性范围为8.5524~85.5240μg/mL,平均回收率为98.32%。结论 HPLC法操作简便快速,结果准确,重现性好,可用于双藤痹痛凝胶膏剂的质量控制。Objective To control the quality of Shuangteng Bitong cataplasm.Methods The contents of Triptolide and Sinomenine in Shuangteng Bitong cataplasm were determined by HPLC.Results The linear range of Triptolide was 1.5216-15.2160 μg·mL-1,and the average recovery was 98.07%.The linear range of Sinomenine was 8.5524-85.5240 μg·mL-1,and the average recovery was 98.32%.Conclusion This method is simple,rapid,accurate,reproducible,and could be used for the quality control of Shuangteng Bitong cataplasm.

关 键 词：双藤痹痛凝胶膏剂 雷公藤甲素 青藤碱 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]