栀子绿原酸含量的紫外-分光光度法测定  

Determination of Chlorogenic Acid in Gardenia Jasminoides Ellis by U-Vspectrophotometry

作　　者：陈亮[1] 林培玲[1] 丁春花[1] 马斌乐 梁一池[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《福建中医药大学学报》2013年第5期36-37,共2页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省科技厅重大项目(2011Y4005)

摘　　要：目的建立栀子绿原酸含量的测定方法。方法采用紫外-分光光度法,以50%甲醇为空白,在波长327 nm处测定绿原酸含量。结果绿原酸在3.96-10.56μg线性良好,平均加样回收率为101.39%,RSD为1.71%。结论紫外-分光光度法操作简便、准确、重复性好,可作为栀子质量控制的检测方法。Objective To establish a method for the determination of chlorogenic acid in gardenia jasminoides ellis.Methods The content of chlorogenic acid was detected by using UV-spectrophotometry,50% methanol is used as reference substance and the detective wavelength was 327 nm.Results The linear range of chlorogenic acid was 3.96-10.56 μg,the average recovery was 101.39%(RSD=1.71%).Conclusion UV-spectrophotometry is simple,accurate and reproducible,and it can be used for the quality control of gardenia jasminoides ellis.

关 键 词：栀子 绿原酸 紫外-分光光度法 

分 类 号：R284.1[医药卫生—中药学]