丹参酚酸B盐联合黄芪多糖预处理对大鼠脑缺血再灌注细胞凋亡及GFAP表达的影响  

Effects of Salvianolic Acid B Combined Astragalus Polysacharin Preconditioning on Apoptosis of Neuronal Cells and Expression of GFAP after Focal Cerebral Ischemia-Reperfusion in Rats

作　　者：武欣[1] 赵宝民[2] 李炜[3] 

机构地区：[1]河北北方学院病理教研室,河北张家口075000 [2]河北北方学院附属第一医院老年病科,河北张家口075000 [3]河北北方学院药学系,河北张家口075000

出　　处：《河北北方学院学报（自然科学版）》2013年第4期59-62,共4页Journal of Hebei North University:Natural Science Edition

摘　　要：目的观察丹参酚酸B盐联合黄芪多糖预处理对SD大鼠脑缺血再灌注损伤细胞凋亡及脑组织中胶质纤维酸性蛋白(GFAP)表达的影响,探讨该预处理方式对脑缺血再灌注损伤后梗死灶及缺血半暗带重塑的影响。方法以线栓法建立缺血再灌注模型——大脑中动脉梗塞模型(MCAO)。将138只雄性SD大鼠随机分为6组(各23只):正常组、假手术组、模型(缺血再灌注)组、丹参酚酸B盐组、黄芪多糖预处理组及丹参酚酸B盐联合黄芪多糖预处理组。观察丹参酚酸B盐联合黄芪多糖预处理对大鼠脑缺血再灌注神经功能、脑梗死面积和病理形态学的影响。采用TUNEL法检测缺血半暗带和灶中心区凋亡细胞,采用免疫组化法检测缺血半暗带和灶中心区GFAP表达的变化。结果模型组与假手术组相比,缺血半暗带凋亡细胞及GFAP阳性细胞数目增多;丹参酚酸B盐联合黄芪多糖预处理可不同程度地降低实验性脑缺血大鼠的神经功能评分(Longa评分)、减小梗死灶面积并使脑组织病理形态改变减轻(P<0.05);丹参酚酸B盐联合黄芪多糖预处理组大鼠脑组织缺血半暗带凋亡细胞和GFAP表达减少,而梗死灶中心凋亡细胞和GFAP表达却增多。结论丹参酚酸B盐联合黄芪多糖预处理可能通过调节脑缺血再灌注损伤细胞凋亡和GFAP表达而促进脑重塑。Objective To observe the impacts of salvianolic acid B(SA-B)combined astragalus polysacharin(aps)preconditioning on apoptosis of neuronal cells and expression of GFAP after focal cerebral ischemia-reperfusion,while investigating the effects on remodeling in ischemic penumbra and ischemic focus center.Methods The string-fastening method was used to establish focal ischemia-reperfusion models in SD rats and one hundred and thirty-eight SD male rats were randomly divided into six groups:normal(n=23),shamop-erated group(n=23),model(ischemia and reperfusion)group(n=23),salvianolic acid B(SA-B)preconditioning group(n=23),astragalus polysacharin(aps)preconditioning group(n=23),salvianolic acid B(SA-B)combined astragalus polysacharin(aps)preconditioning group(n=23).The effects of salvianolic acid B(SA-B)combined astragalus polysacharin(aps)preconditioning were observed on neuralogical function,area of cerebral infarction and pathomorphology of brain tissues,after focal cerebral ischemia-reperfusion.The method of TUNEL was used to measure level of apoptosis of neuronal cells,and immunohistochemistry was used to measure level of expression of GFAP in ischemic penumbra and ischemic focus center.Results In the model group,the number of apoptosis of neuronal cells and expression of GFAP increased markedly in ischemic penumbra compared with that in sham-operated group,the score of neurological function of experimental cerebral ischemia rat and area of cerebral infarction were decreased to various degrees,and degree of pathomorphological change of brain tissues was lessened in salvianolic acid B(SA-B)combined astragalus polysacharin(aps)preconditioning group(P&lt;0.05).The apoptosis of neuronal cells and expression of GFAP were less in ischemic penumbra.Meanwhile,the apoptosis of neuronal cells and expression of GFAP increased in infarction area in salvianolic acid B(SA-B)combined astragalus polysacharin(aps)preconditioning group.Conclusion salvianolic acid B(SA-B)combined astragalus polysacharin(aps)preconditioning could promo

关 键 词：丹参酚酸B盐 黄芪多糖 预处理 凋亡 胶质纤维酸性蛋白 大脑中动脉梗塞模型 缺血再灌注 

分 类 号：S[农业科学]