款冬花多糖对肺腺癌A549细胞生长及凋亡的影响  

Effects of Polysaccharides in Tussilago farfara on Growth and Apoptosis of A549 Cells

作　　者：罗强[1] 李迎春[2] 任鸿[3] 孙黎[1] 郭春燕 王晖[4] 

机构地区：[1]河北北方学院生命科学研究中心,河北张家口075000 [2]河北北方学院中医学院,河北张家口075000 [3]河北北方学院附属第一医院神经内科,河北张家口075000 [4]河北北方学院药学系,河北张家口075000

出　　处：《河北北方学院学报（自然科学版）》2013年第4期63-66,共4页Journal of Hebei North University:Natural Science Edition

基　　金：河北省医学科学研究重点课题计划(No.20090588)

摘　　要：目的观察款冬花多糖对人非小细胞肺癌A549生长及凋亡的影响,探讨其体外的抗肿瘤效应。方法体外培养A549细胞,以不同浓度的款冬花多糖为实验组,并设对照组,CCK8法测细胞增殖抑制效应、荧光倒置显微镜观察药物对细胞凋亡的影响,流式细胞术检测A549细胞中P53和Bcl-2蛋白表达情况。结果不同浓度款冬花多糖作用A549细胞24h增殖抑制明显(P<0.05),其抑制效应具有剂量依赖的特点并可诱导A549细胞凋亡,同时上调p53基因表达、下调bcl-2基因表达(P<0.05)。结论款冬花多糖可抑制A549细胞的生长且增殖抑制效应具有剂量依赖性,并可诱导肿瘤细胞凋亡,其抗肿瘤效应可能与细胞凋亡相关基因表达的调控有关。Objective To investigate the effects of polysaccharides in Tussilago farfara on growth and apoptosis of human non-small cell lung cancer A549and explore its anti-tumor effect in vitro.Methods As experimental group,A549cells were cultured in vitro and treated by different concentrations of polysaccharides in Tussilago farfara,while control group was set.The inhibition effectiveness of cell proliferation was detected by CCK-8method,as well as fluorescence inverted microscope was used to observe effects of drugs on cell apoptosis.At the same time,cell apoptosis,protein expressions of p53and bcl-2 were detected by FCM.Results The proliferation of A549cell was inhibited obviously by polysaccharides in Tussilago farfara with different concentrations(P&lt;0.05),and inhibitory effect was in dose-dependent manner.A549cell apoptosis was induced,and gene expression of p53 was up-regulated and bcl-2 down-regulated(P&lt;0.05).Conclusion Polysaccharides in Tussilago farfara could inhibit A549cell growth in dose-dependent manner and induce apoptosis of tumor cells.Its anti-tumor effect could be related with regulation of apoptosis-related genes expression.

关 键 词：款冬花 多糖 A549 CCK-8 P53 BCL-2 

分 类 号：S[农业科学]