亚硒酸钠对小鼠附植前胚胎发育能力的影响  

Effect of Sodium Selenite on the Developmental Ability of Pre-implantation Embryos in Mouse

作　　者：孙洪亮 蔡强 劳艳萍 田明明 柯浩 高亚可 李楠 陆凤花 石德顺 

机构地区：[1]广西亚热带农业生物资源保护与利用国家重点实验室,南宁530005

出　　处：《湖北农业科学》2013年第1期127-130,共4页Hubei Agricultural Sciences

基　　金：国家"863"计划项目(2011AA100607);国家自然科学基金项目(31072033);教育部高等学校博士学科点博导基金项目(20114501110001);广西杰出青年基金项目(2012GXNSFFA060004);广西研究生教育创新计划资助项目(GXU11T31071)

摘　　要：为探讨不同浓度的亚硒酸钠对小鼠体重、超排效果及其胚胎后续发育的影响,将6～7周龄的小鼠随机分为4组,1组为对照组,其余3组按小鼠每千克体重分别注射0.30 mg(低剂量组)、1.50 mg(中剂量组)和2.25 mg(高剂量组)的亚硒酸钠溶液,再分别测量各组小鼠的体重及观察、记录各组小鼠的超排效果和胚胎体外发育情况。结果显示:(1)高剂量组小鼠体重变化量(-0.31 g)显著低于低剂量组(7.95 g)、中剂量组(8.65 g)和对照组(11.06 g)(P<0.05),而低剂量组、中剂量组与对照组之间无显著差异(P>0.05);(2)不同剂量亚硒酸钠处理的小鼠经超排后回收的平均可用胚胎数,各组之间相比均无显著差异(P>0.05),但高剂量组冲出的胚胎经体外培养后的囊胚发育率(17.6%)显著低于对照组(27.6%)(P<0.05),而与低剂量组(24.3%)和中剂量组(20.6%)差异不显著(P>0.05)。以上结果表明,高剂量的亚硒酸钠不利于小鼠胚胎的发育,其作用机制需要从细胞和分子水平做更深入的研究。To examine the effect of sodium selenite in different concentration on the body weight,superovulation and embryonic developmental competency in mouse,the 6～7 weeks-age mice were randomly divided into four groups and treated with different concentration of sodium selenite solution,such as 0(control group),0.30 mg/kg(low dose group),1.50 mg/kg(medium dose group),2.25 mg/kg(high dose group),respectively.And then the body weight of mice was measured,as well as the number of embryos derived from superovulation and the in vitro developmental rate of embryo was recorded in each group.The results showed that the change of the mice body weight in high dose group(-0.31 g) was significantly lower than those of other three groups(control: 11.06 g,low: 7.95 g,medium: 8.65 g,P&lt;0.05),but there was no remarkably different among the low dose group,medium dose group and control group(P&gt;0.05).There was no significant difference in the number of embryos derived from mice superovulation among the four groups(P&gt;0.05).Compared with the control group,the blastocyst developmental rate of embryo from superovulation by in vitro culture was significantly decreased in high dose group(17.6% vs 27.6%,P&lt;0.05),but it had no significant difference with the low dose group(24.3%) and medium dose group(20.6%)(P&gt;0.05).These results indicated that high doses of sodium selenite had toxicity on embryonic developmental ability,and its mechanism should be took an intensive study at the cellular and molecular level.

关 键 词：小鼠 亚硒酸钠 胚胎发育 

分 类 号：S[农业科学]