B6-Co与B6小鼠眼睑角质形成细胞的原代无血清培养及比较研究  

Serum-free Culture and Comparative Study of B6-Co and B6 Murine Eyelid Primary Keratinocytes

作　　者：卢泽艳[1] 郑良凤[1] 李瑶 邵义祥 

机构地区：[1]南通大学实验动物中心,江苏南通226001 [2]南通大学比较医学研究所,江苏南通226001

出　　处：《湖北农业科学》2013年第2期393-397,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30671081);江苏省自然科学基金项目(BK2010279)

摘　　要：为进一步研究B6-Co小鼠眼睑发育缺陷机制,首次培养了小鼠眼睑角质形成细胞,并对B6-Co和B6小鼠眼睑角质形成细胞的迁移能力进行划痕实验分析,FITC-phalloidin染色比较B6-Co和B6小鼠肌动蛋白细胞骨架。结果表明,B6-Co小鼠眼睑角质形成细胞迁移的数目和距离明显少于B6小鼠,细胞骨架异常,B6-Co小鼠眼睑闭合不全(EOB)是由小鼠眼睑角质形成细胞迁移缺陷造成的。For further study of the mechanism of the eyelid development defects,mouse eyelid primary keratinocytes were cultured for the first time,the motility of keratinocytes were analyzed by in vitro migration assay.The cytoskeleton of B6-Co and B6 mice actin were compared by FITC-phalloidin staining.The results showed that there was significant reduction of the keratinocyte motility after scratch in the cells from the B6-Co mice and abnormal cytoskeleton,suggesting that a defect of cell migration caused by abnormal cytoskeleton structure leads to the failure of embryonic eyelid closure in B6-Co mice.

关 键 词：小鼠 眼睑闭合不全(EOB) 角质形成细胞 肌动蛋白细胞骨架 无血清培养 

分 类 号：S[农业科学]