花生自身基因组原位杂交分析  

Self-genomic in situ Hybridization Analysis of Peanut

作　　者：杨飞[1] 龙舟锴 徐延浩[2] 张文英[2] 

机构地区：[1]长江大学医学院,湖北荆州434025 [2]长江大学农学院,湖北荆州434025

出　　处：《湖北农业科学》2013年第3期527-529,537,共4页Hubei Agricultural Sciences

基　　金：湖北省自然科学基金项目(2010CDB04403);湖北省教育厅科学技术研究项目(Q20101320)

摘　　要：利用自身基因组荧光原位杂交技术,对花生(Arachis hypogaea L.)进行自身基因组原位杂交分析。结果显示,杂交信号沿所有染色体的全长分布,染色体着丝粒区、近着丝粒区和部分DAPI深染的区域存在强烈的杂交信号,染色体远端的杂交信号偏弱,染色体上存在少数未观察到杂交信号的DAPI深染区域。花生自身基因组原位杂交存在明显的非均匀染色体杂交带型,这说明基因区成簇分布在小的染色体区域并被重复序列间隔开。Self-genomic in situ hybridization(self-GISH) analysis was performed on peanut(Arachis hypogaea L.) metaphase chromosome and interphase nucleus.The results showed that hybridization signals were dispersed along the chromosome length.The strong signals were mostly detected in the centromeric,pericentromeric and some of the DAPI positive regions.The weak signals were observed in the distal of chromosome regions.Some DAPI positive regions were not labeled with any signal.The non-uniform self-GISH pattern of peanut indicated that the gene rich clusters were located at small regions of chromosome and disrupted by large segmental of repetitive DNA.

关 键 词：花生(Arachis HYPOGAEA L.) 自身基因组原位杂交 重复DNA序列 基因组组织 

分 类 号：S[农业科学]