TMV溶液诱导蚕豆根尖微核的研究  

Study on Micronucleus Rates of Vicia Faba Root Tips Induced by TMV Solution

作　　者：刘开全[1] 马学萍[2] 宋路平 李波[2] 

机构地区：[1]曲靖师范学院化学化工学院,云南曲靖655011 [2]曲靖师范学院生物资源与环境科学学院,云南曲靖655011

出　　处：《湖北农业科学》2013年第3期561-563,共3页Hubei Agricultural Sciences

基　　金：曲靖师院青年科研基金项目(2008QN033)

摘　　要：应用蚕豆根尖微核试验初步研究了一定浓度的烟草花叶病毒(Tobacco Mosaic Virus,TMV)溶液对环境污染的效应。结果表明,TMV溶液浓度与各处理蚕豆根尖微核率的相关性较强(r=0.982),且差异极显著(P=0.003<0.01),即蚕豆根尖微核率随着TMV溶液浓度的升高而增加;同一浓度的TMV溶液处理蚕豆根尖不同时间后其微核率在各组间没有显著差异(P>0.01),且蚕豆根尖微核率与处理时间相关性不强(r=0.312)。研究表明蚕豆根尖细胞微核技术可应用于烟草花叶病毒遗传毒性监测。In order to preliminary studying on effect of TMV solution to environmental deterioration,Micronucleus tests were conducted in Vicia faba root tips treated with different concentrations of TMV solution in the study.The results as follows: TMV solution had some inductive effect on micronucleus of Vicia faba root tips.Compared with the control group,the difference was very significant between different concentration of TMV solution and micronucleus rate of Vicia faba root tips(P= 0.003&lt;0.01).Its micronucleus rate tended to increase with the increasing of TMV solution concentration(r=0.982).Comparing with the control group,the difference was not significant(P&gt;0.01,r=0.312) between different processing time and micronucleus rate of Vicia faba root tips treated by the same concentration of TMV solution.It is suggested that micronucleus test can be applied to monitor inherent toxicity of tobacco mosaic virus.

关 键 词：烟草花叶病毒(TMV) 蚕豆根尖 微核率 

分 类 号：S[农业科学]