烟草靶斑病菌毒素对烟草防御酶及丙二醛含量的影响  

Effects of Crude Toxin from Rhizoctonia solani on Defensive Enzyme Activity and MDA Content in Tobacco Seedling

作　　者：伏颖[1] 赵艳琴[1,2] 陈建光[1] 赵秀香[1] 吴元华[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866 [2]内蒙古民族大学农学院,内蒙古通辽028043

出　　处：《湖北农业科学》2013年第3期575-577,共3页Hubei Agricultural Sciences

基　　金：国家烟草专卖局科技攻关项目(国烟办综[2010]182号);辽宁省烟草专卖局科技攻关项目(辽烟计[2010]86号)

摘　　要：采用烟草靶斑病菌(Rhizoctonia solani)粗毒素原液处理6叶期烟草幼苗,测定12、24、36、48、60、72h后烟草叶片中过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)的活性和丙二醛(MDA)含量。结果表明,经烟草靶斑病菌粗毒素处理后的烟草POD、PAL的活性及MDA含量都高于对照,并呈现一定的波动性;PPO和CAT先升高后下降,始终高于对照;而SOD酶的活性表现为持续下降。Tobacco seedlings at 6-leaf stage were treated with crude toxin from tobacco target spot disease(Rhizoctonia solani),of which the peroxidase(POD),polyphenol oxidase(PPO),superoxide dismutase(SOD),catalase(CAT) and phenylalanine ammonialyase(PAL) activity as well as malondialdehyde(MDA) content was measured 12,24,36,48,60,72 h after treatment.The results demonstrated that after toxin treatment,POD,PAL and MDA were activated as their activities were higher than control,changes of POD,PAL and MDA were undulate;PPO and CAT activities increased firstly and then decreased along with time increasing,but always higher than control;SOD activity increased continually.

关 键 词：烟草靶斑病菌(Rhizoctonia solani) 毒素 烟草 防御酶 

分 类 号：S[农业科学]