烟草疫霉分离及生长培养基的选择  

Selection of Isolating and Culturing Medium for Phytophthora nicotianae

作　　者：苏凯[1] 桑维钧[1] 张新强 王慧[1] 

机构地区：[1]贵州大学农学院/贵州山地农业病虫害重点实验室,贵阳550025 [2]甘肃省天水市秦州县林业局,甘肃天水741000

出　　处：《湖北农业科学》2013年第3期708-710,共3页Hubei Agricultural Sciences

基　　金：中国烟草总公司贵州省公司项目(200901)

摘　　要：采用不同的基本培养基添加抗生素组合对烟草疫霉(Phytophthora nicotianae)进行分离培养。结果表明,以V8培养基(100 mL/L V8汁+0.02 g/L CaCO3+2 g/L洋菜)为基本培养基附加10 mg/mL氨苄青霉素+5 mg/mL制霉素+5 mg/mL多菌灵的选择培养基分离烟草疫霉的效果较好,分离成功率达100%,菌丝的生长状态也较好。菌丝分离后在V8基本培养基中培养,菌丝紧密、浓白、粗壮,菌落大、长势旺。Different base medium adding different combination of antibiotics were applied for isolation and culture of Phytophthora nicotianae.The results showed that selective medium with V8 base medium(100 mL/L V8juice+0.02 g/L CaCO3+2 g/L agar) adding 10 mg/mL ampicillin +5 mg/mL nysfungin+5 mg/mL carbendazim had the best isolation effect as the successful isolation rate was 100%,and the hypha grew well on this medium.After separation,the hypha could be cultured in V8 base medium as the hypha was white,tight and strong with big bacteria clone and strong growth potential.

关 键 词：烟草疫霉(Phytophthora nicotianae) 培养基 分离 生长 

分 类 号：S[农业科学]