体外产气法和尼龙袋法测定饲料营养成分的比较  

Comparison of in vitro Gas Production and in situ Nylon Bag Technique in Determining the Nutrient of Different Feedstuffs

作　　者：杜晋平[1] 殷裕斌[1] 李助南[1] 

机构地区：[1]长江大学动物科学学院,湖北荆州434025

出　　处：《湖北农业科学》2013年第4期923-926,共4页Hubei Agricultural Sciences

基　　金：长江大学博士科研启动基金资助项目(博科启110926)

摘　　要：以玉米、豆粕、啤酒糟和玉米秸青贮为对象,分别测定了每种饲料的体外产气参数和瘤胃尼龙袋发酵参数,比较2种方法测定饲料营养成分发酵的异同及相互关系。结果表明,2种方法在评价玉米干物质(Dry matter,DM)和有机物(Organic matter,OM),豆粕DM,啤酒糟OM 48 h消失率方面存在显著差异(P<0.05),在玉米、豆粕、啤酒糟的其余成分和玉米秸青贮各成分方面差异不显著;玉米和豆粕DM和OM的快速降解部分比例(a)较高,因而2种方法的体外产气速率(C)也快;玉米DM和OM有较高的可发酵成分(a+b),因而其48 h产气量(Y)最高;豆粕较高的粗蛋白(Crude protein,CP)导致其体外产气量最低;对低纤维饲料来说,DM和OM c值高者具有较高的产气速率和较小的延滞期,而对高纤维饲料来说,造成较高的产气速率和较小延滞期的主要原因可能来自于高的OM c值而非高的DM c值;影响理论最大产气量(B)的最主要因素是中性洗涤纤维,其次是酸性洗涤纤维。The difference and relationship of four feedstuffs nutrients’ fermentation parameters determined by in vitro gas production technology(IVGPT) and in situ nylon bag technique(ISNBT) were evaluated.Taking the corn,soybean meal(SBM),brewers’ grains(BG),silage maize straw(SMS) as test materials,parameters of each feed determined by IVGST and ISNBT were measured,respectively.There were significant differences(p&lt;0.05) in rumen degradability of corn’s dry matter(DM) and organic matter(OM),SBMs’OM,and BGs’OM.However,there was no significant difference in the parameters of others nutrients of the four feedstuffs.In vitro gas production rate constants(GPRC,C) for DM and OM in corn and SBM were faster,resulting from the higher rapid degradation percentage(a),than others.The most cumulative gas production(CGP) within 48 h(Y) was observed in corn feedstuff because of its higher biodegradable ingredients(a+b) than others.In contrast,the least CGP emerged in SBM feedstuff due to its highest content of crude protein(CP) than others.For low-fiber feedstuffs,the higher degradation rate constant(c) of slow degradation parts(b) of DM and OM corresponded to the faster GPR and less lag time.On the contrary,it was showed that only the higher degradation rate constant of OM resulted in the same result for high-fiber feedstuffs.The two most important influencing factors on theoretical maximum gas production were acid detergent fiber(ADF) and neutral detergent fiber(NDF) content of feedstuffs.

关 键 词：体外产气法 瘤胃尼龙袋法 降解率 发酵参数 产气参数 

分 类 号：S[农业科学]