植物激素对灵菊七愈伤组织中黄酮含量的影响  

Effect of Plant Hormones on Flavones Content in Callus of Gynura medica

作　　者：范三微[1] 董丽辉[1] 凌庆枝[1] 屠晓玮[1] 柯吴琦[1] 高莉莉[1] 魏兆军[2] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100 [2]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《湖北农业科学》2013年第6期1450-1452,1456,共4页Hubei Agricultural Sciences

基　　金：浙江省中医药科技计划项目(2011ZB132);浙江省大学生科技创新计划项目(ZJRCKJCX201007)

摘　　要：以灵菊七(Gynura medica)无菌试管苗叶片为外植体,接种于愈伤组织诱导培养基上,诱导形成愈伤组织,并进行愈伤组织继代培养。将继代培养的愈伤组织接种于含有不同激素(浓度)组合的愈伤增殖培养基上,以硝酸铝显色法测定其中黄酮的含量。结果表明,在4种愈伤组织增殖培养基上,愈伤组织中总黄酮含量最大值分别达到0.794%、2.112%、1.652%、2.792%。培养基MS+0.5 mg/L 6-BA+0.5 mg/LNAA+1.0 mg/L GA最适用于灵菊七愈伤组织中黄酮总量的积累。The leaves collected from aseptic test-tube plantlet of Gynura medica were cut and cultured on medium to induce callus,and then regenerated the callus on the same medium.Callus regenerated was inoculated on 4 medium containing different hormones for propagation,and determined the content of total flavones by using Al(NO3)3 colorimetry.The results showed that the maximum total flavones content were up to 0.794%,2.112%,1.652%,2.792% on 4 medium respectively.MS+0.5 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA was the optimum medium for flavones accumulating.

关 键 词：灵菊七(Gynura medica) 愈伤组织 植物激素 黄酮 

分 类 号：S[农业科学]