转OsCYP2基因水稻后代的遗传分析及农艺性状观察  

Genetic Analysis and Agronomic Characters Observation on Transgenic OsCYP2 Rice

作　　者：闫海生[1] 雷闯[1] 马凌霄[1] 刘红双[1] 马景勇[1] 

机构地区：[1]吉林农业大学农学院,长春130118

出　　处：《湖北农业科学》2013年第7期1510-1511,1536,共3页Hubei Agricultural Sciences

基　　金：吉林省科技支撑计划项目(2007227);长春市科技支撑计划项目(09KZ28)

摘　　要：通过对转OsCYP2基因水稻(Oryza stativa L.)吉农大30后代的PCR检测,发现转基因后代中大部分株系分离比率符合3:1的遗传比率,依此推断该部分植株中插入片段的遗传分离方式符合孟德尔遗传规律;对转基因植株及对照植株(吉农大30)的农艺性状进行观察比较后发现转基因植株的平均穗长、穗粒数、结实率、株高以及千粒重等性状表现低于对照,但是分蘖数明显增加,生育期延长,而且转基因株系之间变异性较大。According to PCR identification,most posterity lines of transgenic OsCYP2 rice Jinongda 30 fit the 3∶1 Mendelian segregation pattern,it was inferred that the short insertions could inherit stably by the way of Mendelian inheritance.Observation and comparison of the agronomic tracts of these line showed that the spike length,grain number,plant height and 1 000 kernel weight was lower than those of the control group;while the tiller number was much more than the control group;the growth period extended and the differences between the transgenic lines was big.

关 键 词：水稻(Oryza stativa L.) 转基因 OsCYP2基因 遗传分离比例 农艺性状 

分 类 号：S[农业科学]