高效液相色谱法测定鱼、虾和鸡肉中的呋喃唑酮残留  

Determination of Furazolidone Residue in Fish, Shrimp and Chicken by HPLC

作　　者：余晓[1] 苏子豪[1] 翟海云[1] 卢绮雯[1] 潘育方[1] 

机构地区：[1]广东药学院,广州510006

出　　处：《湖北农业科学》2013年第16期3974-3976,共3页Hubei Agricultural Sciences

基　　金：广东省科技计划项目(2010B020316010)

摘　　要：建立高效液相色谱(HPLC)法测定鱼、虾和鸡肉中呋喃唑酮药物残留量的方法。色谱条件为Kromasil C18(250 mm×4.6 mm,5μm)柱,流动相乙腈-0.1%磷酸水溶液(30∶70,V/V),流速1.0 mL/min,检测波长365 nm,柱温25℃。结果表明,呋喃唑酮标准溶液在0.2~5.0 ng范围内与峰面积呈良好的线性关系(r=0.999 1),检出限为0.2μg/kg(S/N=3)。样品平均加标回收率为97.7%~102.9%,RSD为1.1%~1.5%(n=6)。该方法简单、快速、灵敏度高、重复性好,适用于鱼、虾和鸡肉中呋喃唑酮的残留分析。The HPLC method for detecting furazolidone residue in fish, shrimp and chicken samples was established. The sample was seperated in the Kromasil C18column(250 mm×4.6 mm,5 μm) with acetonitrile-0.1% phosphoric acid solution(30∶70, V / V) at 25 ℃; the flow rate was 1.0 mL / min. The detecting wavelength was set at 365 nm. The results showed that the linear range of furazolidone was 0.2~5.0 ng(r=0.999 1), the LOD was 0.2 μg / kg(S/N=3). The average recovery was 97.7%~102.9% with the RSD of 1.1~1.5%(n=6). In conclusion, this simple, rapid, sensitive and reproducible method was suitable for analyzing the furazolidone residue in chicken, fish and shrimp.

关 键 词：呋喃唑酮 药物残留 高效液相色谱法 

分 类 号：S[农业科学]