响应面法优化桂花总黄酮提取工艺  

Optimization of Extraction Process of Flavonoids from Osmanthus fragrans Using Response Surface Method

作　　者：史玉敏[1] 严恒 蔡朝晖[1] 陈洪国[1] 

机构地区：[1]湖北科技学院核技术与化学生命科学学院,湖北咸宁437000 [2]湖北省食品质量监督检验研究中心,武汉430030

出　　处：《湖北农业科学》2013年第17期4189-4192,共4页Hubei Agricultural Sciences

基　　金：湖北省自然基金重点项目(2011CDA063);湖北省教育厅重点项目(CXY2009B043);湖北省高等学校青年教师深入企业行动计划项目(XD20100662);咸宁市科学技术研究项目(No.2010018)

摘　　要：以桂花(Osmanthus fragrans)干粉为原料,采用索氏提取法从中提取总黄酮,考察乙醇溶液体积分数、液料比(V乙醇∶m桂花粉,mL/g)、提取时间对桂花中总黄酮提取率的影响,并利用Box-Behnken试验设计进行响应面分析。结果表明索氏提取法提取桂花总黄酮的最优工艺条件为乙醇溶液体积分数83%、液料比29.8∶1、提取时间3.1 h,此条件下桂花中总黄酮提取率可达19.1%。Total flavonoids were extracted from flower of Osmanthus fragrans by Soxhlet extraction method.The effects of volume fraction of alcohol,liquid to solid ratio(Valcohol ∶mosmanthus fragrans,mL/g),extraction time on yield of flavonoids were studied.Response surface method was employed to optimize the extraction process.Results showed that the optimum conditions for Soxhlet extraction of total flavonoids were alcohol volume fraction,83%;liquid to solid ratio,29.8∶1;extraction time,3.1 h.Extraction yield of total flavonoids was 19.1% under these conditions.

关 键 词：桂花(Osmanthus fragrans) 总黄酮 索氏提取 响应面法 

分 类 号：S[农业科学]