紫外分光光度-比色法检测柿蒂中三萜类化合物的含量  

Detection of Triterpenoid Content in Persimmon Calyx by Ultraviolet Spectrophotometry-colorimetry

作　　者：蒋莺[1] 钟晓红[1] 陆英[1] 程建宇[1] 张双[1] 何艳君[1] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128

出　　处：《湖南农业科学》2013年第5期81-84,共4页Hunan Agricultural Sciences

基　　金：长沙市科技局项目资助(1016:DD0105)

摘　　要：对紫外分光光度-比色法检测柿蒂三萜类化合物含量的条件进行了优化,并对不同品种不同采收时期柿蒂中的三萜类化合物含量进行了检测。结果表明:最佳的比色条件为5%香草醛-冰醋酸0.4 mL,高氯酸0.8 mL,在20～60 min内测定吸光度,此时相对标准偏差RSD为1.66%,吸光度较稳定,精密度好。对不同采收时期不同品种柿蒂中三萜类化合物含量的检测结果表明,所有采收品种在5月时三萜类化合物含量最高,随着生长期的延长均呈下降趋势,同一时期不同品种间三萜类化合物含量相差较大,三代斤、水柿和牛心柿不同采收时期三萜类化合物含量均较高,次郎含量最低。The conditions for detecting triterpenoid content in persimmon calyx by ultraviolet spectrophotometry colorimetry were optimized,and the triterpenoid content in different varieties of persimmon calyx in different harvest periods was detected.The results showed that the optimal colorimetry conditions were 0.4 mL of 5% vanillin-glacial acetic acid,0.8 mL of perchloric acid,detecting absorbency within 20-60 min.With the optimal colorimetry conditions,the RSD was 1.66%,the absorbency was relatively stable,and the precision was great.The testing results for triterpenoid content in of different varieties persimmon calyx in different harvest periods showed that all of the harvested varieties had the highest triterpenoid content in May;the triterpenoid content showed a decreasing trend with growth period developed;the triterpenoid content had the hug difference between different varieties at the same harvest period;the triterpenoid content in Sandaijin,Shuishi and Niuxinshi in different harvest periods all was relatively high,and that in Cilang was the lowest.

关 键 词：柿蒂:紫外分光光度法:三萜类化合物含量 

分 类 号：S[农业科学]