桑青枯雷尔氏菌鞭毛蛋白flic基因的克隆和序列分析  

Cloning and Sequence Analysis of the Flagellin Gene flic in Ralstonia Solanacearum

作　　者：杨金宏[1] 陈冰洁[2] 程煜[2] 孔卫青[1] 

机构地区：[1]安康学院陕西省蚕桑重点实验室,陕西安康725007 [2]陕西省安康中学,陕西安康725000

出　　处：《湖南农业科学》2013年第6期4-5,8,共3页Hunan Agricultural Sciences

基　　金：陕西省科技厅农业攻关项目(2011K01-40);陕西省"春笋计划"课题研究项目

摘　　要：以桑青枯雷尔氏菌MR111为材料,对MR111的鞭毛蛋白基因flic的部分序列进行了PCR扩增和测序。结果表明:获得了flic基因400 bp的序列,序列编码蛋白有典型的鞭毛蛋白N-末端的螺旋区。NCBI在线BLAST分析表明,该序列与其他青枯雷尔氏菌菌株的鞭毛蛋白基因的同源性在97%以上。PCR amplification and sequencing analysis were conducted to partial sequence of flagellin gene flic in Ralstonia solanacearum MR111.The results indicated that 400 bp of flic gene sequence was obtained,and a typical spiral area of flagellin lied in the N-terminal of sequence coding protein.Moreover,the NCBI online BLAST analysis indicated that the homology between this sequence and flagellin gene from the other strains of Ralstonia solanacearum reached over 97%.

关 键 词：青枯雷尔氏菌 鞭毛蛋白基因 克隆 序列分析 

分 类 号：S[农业科学]