甘蓝型油菜pol-CMS杂交种纯度SSR鉴定与田间鉴定单株吻合率研究  

SSR Identification of Purity for pol-CMS Hybrid Seeds of Brassica napus and Field Identification of Its Coincidence Rate of Single Plant

作　　者：王同华[1] 陈卫江[1] 王建喜[1] 

机构地区：[1]湖南省作物研究所,湖南长沙410125

出　　处：《湖南农业科学》2013年第10期4-6,13,共4页Hunan Agricultural Sciences

基　　金：国家863项目(2011AA10A104);湖南省农业科学院科技创新项目(2010hnnkycx07)

摘　　要：为探讨SSR标记技术在甘蓝型油菜pol-CMS杂交种纯度鉴定中的准确性,研究采用SSR标记技术和田间种植鉴定两种方法对3个推广面积较大的油菜杂交种样品纯度进行一一对比分析,结果表明:两种鉴定方法的鉴定偏差在2%以内,单株鉴定结果吻合率在97%以上,表明利用SSR标记鉴定甘蓝型油菜杂交种纯度是准确可靠的。In order to discuss the nicety of SSR marker technology in identification of purity for pol-CMS hybrid seeds of Brassica napus, the SSR marker technology and the field planting and identification method were adopted to compare and analyze the purities of three hybrid rape varieties with large extending area. The results showed that the error of the two identification methods was all within 2% and the coincidence rate of single plant was over 97%. Therefore, using SSR marker to identify purity of hybrid seeds of Brassica napus was exact and reliable.

关 键 词：甘蓝型油菜 pol-CMS SSR 田间鉴定 吻合率 

分 类 号：S565.4[农业科学—作物学]