不同浓度硫氢化钠对人脐静脉平滑肌细胞生长的影响  

Effect of Different Concentrations of Sodium Hydrosulfide on Human Umbilical Vein Smooth Muscle Cells

作　　者：昝海英[1] 刘爽[2] 武慧娟[3] 张鸣号 徐华 杨晓玲 贾月霞 曹军 李桂忠 

机构地区：[1]宁夏医科大学,银川750004 [2]山东滨州医学院医学研究中心,烟台264003 [3]宁夏医科大学基础医学院病理生理学系,银川750004

出　　处：《宁夏医科大学学报》2013年第8期856-859,831,共5页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金(NZ09105)

摘　　要：目的摸索体外培养体系中不影响人脐静脉平滑肌细胞(HUVSMCs)生长的硫氢化钠(NaHS)适宜浓度。方法采用原代培养人脐静脉平滑肌细胞,实验分为八组,分别是正常对照组和七个NaHS浓度组,依次为:5.0×10-3M、1.0×10-3M、2.0×10-4M、4.0×10-5M、8.0×10-6M、1.6×10-6M、3.2×10-7M。采用细胞计数法绘制细胞生长曲线并观察细胞的生长状态,MTT检测细胞生长活力,流式细胞术检测细胞周期并进行比较。结果 4.0×10-5M组、8.0×10-6M组、1.6×10-6M组、3.2×10-7M组从细胞生长活力、细胞周期及细胞生长曲线绘制三方面与对照组相比,差异均无统计学意义(P>0.05);5.0×10-3M组、1.0×10-3M组、2.0×10-4M组与对照组相比,细胞生长受到抑制,差异有统计学意义(P<0.05)。结论 1.6×10-6M～4.0×10-5M中任意浓度的NaHS均不影响HUVSMCs的正常生长,其可作为培养HUVSMCs并作为实验材料的浓度范围。Objective To explore the optimal concentration of NaHS for primary cultured human umbilical vein smooth muscle cells( HUSMCs) growing. Methods Culturing human umbilical vein smooth muscle cells were divided into eight different concentrations: normal control group( culture medium without NaHS),5. 0 × 10-3M group( culture medium containing 5. 0 × 10-3M NaHS),1. 0 × 10-3M group( culture medium containing 1. 0 × 10-3M NaHS),2. 0 × 10-4M group( culture medium containing 2. 0 × 10-4M NaHS),4. 0× 10-5M group( culture medium containing 4. 0 × 10-5M NaHS),8. 0 × 10-6M group( culture medium containing 8. 0 × 10-6M NaHS),1. 6 × 10-6M group( culture medium containing 1. 6 × 10-6M NaHS),3. 2 ×10-7M group( culture medium containing 3. 2 × 10-7M NaHS). Cell growth activity was detected by MTT.Cell cycle was determined by flow cytometry and the cell growth curve was draw. Results The cell cycle and the plateau density in 4. 0 × 10-5M,8. 0 × 10-6M,1. 6 × 10-6M,3. 2 × 10-7M showed no different from those in control group( P &gt; 0. 05). Compared with the control group,Cell growth was significantly inhibited in 5. 0 × 10-3M,1. 0 × 10-3M,2. 0 × 10-4M( P &lt; 0. 05). Conclusion 1. 6 × 10-6M ～ 4. 0 × 10-5M of NaHS can be used as the optimal concentration range in HUSMCs growth.

关 键 词：人脐静脉平滑肌细胞 硫氢化钠 细胞培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]