丙泊酚对大鼠局灶性脑缺血再灌注后AQP-4mRNA表达的影响  

Effects of Propofol on Expression of AQP-4 in Rat Models of Transient Focal Ischemia-reperfusion

作　　者：赵玲[1] 盛旭东[2] 明婷[1] 王武涛[1] 李争卫 李静[1] 何爱萍[1] 

机构地区：[1]西安医学院附属医院麻醉科,西安710077 [2]西安医学院附属医院神经外科,西安710077

出　　处：《宁夏医科大学学报》2013年第10期1083-1086,共4页Journal of Ningxia Medical University

基　　金：西安医学院第一附属医院院级课题面上项目(XYFY08-19)

摘　　要：目的探讨丙泊酚对大鼠局灶性脑缺血再灌注后AQP-4mRNA表达的影响。方法健康雄性SD大鼠192只,按随机数字表法分成丙泊酚组(n=64),生理盐水组(NS)(n=64),假手术组(n=64)。采用线栓法制备大鼠局灶性脑缺血再灌注模型,NS、丙泊酚组在阻断大脑中动脉lh后行再灌注,丙泊酚组在缺血前10min腹腔注射丙泊酚10mg/100g,NS组给予等量生理盐水,假手术组只作切口。各组分别在再灌注后3h、1d、3d、7d做神经功能测定,其后将大鼠断头处死,测量脑水含量,用RT-PCR法测定AQP-4mRNA表达水平。结果①与假手术组比较,丙泊酚组术后3h、1d、3d、7d神经功能评分均高于假手术组(P<0.05),与NS组比较,丙泊酚组术后各时间点神经功能评分均低于NS组(P<0.05)。②术后各时间点丙泊酚组与假手术组比较,脑含水量显著增加(P<0.05);与NS组比较,脑含水量显著减少(P<0.05)。与假手术组比较,NS组脑含水量也显著增加(P<0.05)。③丙泊酚组AQP-4 mRNA含量在各时间点表达与NS组比较均较低(P<0.05)。NS组在术后各时间点,AQP-4 mRNA含量均比假手术组高(P<0.05)。结论丙泊酚可下调脑缺血再灌注损伤大鼠脑组织AQP-4mRNA的表达,可能是其减轻大鼠脑缺血再灌注损伤的机制之一。Objective To explore the effect of propofol on the expression of AQP- 4 in rat brains following ischemia- reperfusion injury. Methods 192 healthy SD rats were randomly divided into 3 groups: propofol group B( n = 64),normal saline group NS( n = 64) and sham operation group S( n = 64). A nylon thread( 0. 25 mm in diameter) with rounded tip was inserted at the bifurcation into internal carotid artery and threaded cranially until resistance was felt. In group P propofol 10mg /100g was given intraperitoneally after ischemia- reperfusion. In group NS,NS was given instead of propofol. In group S the carotid arteries were exposed but mid- cerebral artery occlusion was not performed. The animals were killed at 3h,1d,3d,7d after Rosenberg scores following reperfusion was started. Brains were immediately removed and detected the brain water content. The AQP- 4mRNA was assessed by RT- PCR. Results ① The neurological scores in propofol group were higher than that in the sham- operated group( P < 0. 05) at all time points after surgery,and there was obvious difference results between the saline group and propofol group( P < 0. 05). ② Compared with the sham- operated group,the brain water content in propofol group increased significantly( P < 0. 05); and which was lower significantly than that in saline group( P < 0. 05). The levels of the brain water content in saline group were significantly increased compared with the sham- operated group( P < 0. 05). ③ The levels of AQP- 4mRNA expression in propofol group at each time point were lower than that in saline group( P < 0. 05). Compared with saline group,the expressions of AQP- 4mRNA in sham- operated group was significantly decreased( P < 0. 05). Conclusion Propofol can protect the brain from ischemia- reperfusion injure to some extent. The down- regulation of AQP- 4 expression is involved in the mechanism.

关 键 词：丙泊酚 脑缺血 再灌注损伤 水通道蛋白4 

分 类 号：R614.2[医药卫生—麻醉学]