川芎嗪聚乙稀醇药膜预防硬膜外粘连实验研究  

Application of TTPAM in the prevention of laminectomy epidural adhesions

作　　者：魏凌平 冯利刚[2] 刘莹菁 

机构地区：[1]广东省深圳市南山人民医院,深圳518052 [2]广东省深圳市中医院,深圳518020 [3]广东省深圳市宝安中医院,深圳518133

出　　处：《江西医药》2013年第4期302-305,共4页Jiangxi Medical Journal

基　　金：广东省中医药科研资金项目(2010299)

摘　　要：目的探讨自制川芎嗪聚乙稀醇药膜预防椎板切除术后硬膜外粘连的效果。方法将48只成年雄性新西兰白兔随机分为两组,实验组(n=24):行L5椎板切除术并植入自制川芎嗪聚乙稀醇药膜;对照组(n=24):单纯L5椎板切除处。每组分别在术后的第2、4、6、8周随机处死6只,采用改良Rydell标准进行大体观察的粘连分级和计算机图像分析法计算成纤维细胞数以评估瘢痕形成的量。结果术后2周时,两组改良Rydell粘连分级无显著的统计学差异(P=0.18),但是在术后4、6、8周时实验组改良Rydell粘连分级显著低于对照组(P<0.05);实验组硬膜外成纤维细胞数在术后各时间点均显著低于对照组(P<0.05)。结论在兔模型中,川芎嗪聚乙稀醇药膜能有效的减少椎板切除术硬膜外纤维化和瘢痕粘连。Objective To observe the preventive effect of local application of TTPAM The tetramethy lpyrazine polythylene clcohol membrane on epidural adhesion after laminectomy.Methods 48 New Zealand rabbits were randomly divided into two groups(n=24),of which all of the L 5 laminas were resected.TTPAM were placed behind the exposed dura in group B,group A was the control group.Animals were sacrificed respectively in the 2nd week,4th week,6th week and 8th week after the surgery.The sample number was 6 for each group at each time point.We use the modified Rydell standard to evaluate the macroscopical situation of adhesion,the number of fibroblast cells were counted by the way of computer image analysis system.Results The macroscopical detection shows that the grade of group A was higher than the group B by the way of the modified Rydell standard.The microscopical result also demonstrated group B had a lower number of fibroblast to group A.Conclusion Local application of TTPAM can reduce the formation of epidural fibroblast and prevent epidural scar adhesion after laminectomy in the rabbits model it is promising to be applicatd it in clinic.

关 键 词：硬膜外粘连 硬膜外纤维化 椎板切除术 川芎嗪聚乙稀醇薄药膜 

分 类 号：R9[医药卫生—药学]