硫酸铍对小鼠脾脏淋巴细胞增殖及T细胞亚群的影响  

Beryllium Sulfate Effect on Lymphocyte Proliferation and Subgroup of Spleen in Mice

作　　者：严青[1] 刘志宏[2] 龚爱红[1] 张庆锋[1] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学公共卫生学院,银川750004

出　　处：《宁夏医科大学学报》2013年第12期1329-1332,共4页Journal of Ningxia Medical University

基　　金：宁夏高等学校科学技术研究重点项目(NGY2012048);宁夏卫生厅科研项目(2012011)

摘　　要：目的观察硫酸铍(BeSO4)对BALB/C小鼠脾脏淋巴细胞增殖及T细胞亚群的影响。方法 BALB/C小鼠随机分为对照组和BeSO4染毒低(2.5mg·kg-1)、中(5.0mg·kg-1)、高(10.0mg·kg-1)剂量组。每周称量小鼠体重一次,染毒结束后小鼠脱颈椎处死,无菌取出脾脏并称重。MTT法检测小鼠脾脏淋巴细胞增殖,流式细胞术测定T淋巴细胞亚群。结果 BeSO4染毒5周后,低、中、高剂量组BALB/C小鼠体重均下降,其中中、高剂量组与对照组比较差异有统计学意义(P<0.05);低、中、高剂量BeSO4染毒组脾脏重量及脾脏系数均高于对照组(P<0.05);经BeSO4染毒后小鼠脾脏淋巴细胞增殖能力增强,低、中、高剂量组与对照组之间差异有统计学意义(P<0.05);三种剂量BeSO4染毒均可明显增加小鼠脾脏CD4+T细胞数量及CD4+T细胞与CD8+T细胞的比值(P<0.05)。结论 BeSO4可致BALB/C小鼠细胞免疫功能紊乱。Objective To observe the Beryllium sulfate( BeSO4) after the intervention analysis of BeSO4in BALB / C mice spleen lymphocyte proliferation and the lymphocyte subsets. Methods Mice were randomly divided into control group and low BeSO4infected( 2. 5 mg·kg- 1),medium( 5. 0 mg·kg- 1),high dose group( 10. 0 mg · kg- 1). The mice spleen lymphocyte proliferation and T lymphocyte subsets were determined by MTT method and flow cytometry,respectively. Results BeSO4administration after 5 weeks,low,medium and high dose group of BALB / C mice body weight decreased while the middle,high dose group had significant difference compared with control group( P ﹤ 0. 05). Low,medium and high dose spleen weight,spleen coefficient BeSO4group were significantly higher than those of the control group( P ﹤ 0. 05). After BeSO4contaminated mice spleen lymphocyte proliferation ability enhancement,low,medium and high dose group and control group showed significant difference( P ﹤ 0. 05). Three doses of BeSO4could significantly increase mice spleen CD4+T cells and CD4+T cells and the ratio of CD8+T cells( P ﹤ 0. 05). Conclution BeSO4can cause BALB / C mice cellular immune dysfunction.

关 键 词：硫酸铍 淋巴细胞增殖 T细胞亚群 

分 类 号：R114[医药卫生—卫生毒理学]