红菜薹雄性不育系花药败育的细胞形态学观察  

The Cytomorphology Study of Anther Abortion in Male-Sterile Lines of Hong Cai-tai (Brassica campestris L.ssp.chinensis L.var.utilis Tsen et Lee.)

作　　者：田福发[1] 徐跃进[1] 袁黎[1] 陈建军[1] 

机构地区：[1]华中农业大学园艺林学学院,武汉430070

出　　处：《武汉植物学研究》2004年第3期269-272,共4页Journal of Wuhan Botanical Research

基　　金：武汉市科技攻关项目(2002068)资助。

摘　　要：采用石蜡切片技术,在光学显微镜下系统研究了红菜薹(BrassicacampestrisL.ssp.chinensisL.var.utilisTsenetLee.)波里马胞质雄性不育系(PolimaCMS)、红菜薹萝卜胞质雄性不育系(OguraCMS)及相应保持系花药发育过程的细胞形态学特征。观察结果表明:红菜薹PolimaCMS花药发育受阻于孢原细胞阶段,不形成花粉,属无花粉型,此不育系花药不形成绒毡层和中层;而红菜薹OguraCMS花药败育发生于小孢子母细胞期或四分体时期,表现为绒毡层细胞异常,挤压四分体,导致四分体和绒毡层同时解体而败育。Microsporogenesis of Hong Cai-tai's Polima cytoplasmic male-sterile line (Polima CMS),Hong Cai-tai's Ogura cytoplasmic male-sterile line(Ogura CMS) and their maintainers were investigated cytologically by using paraffin section with light microscopy systemly.The observation showed that the anther development of Hong Cai-tai's Polima male-sterile line was blocked without anther at the stage of archesporial cell.The few pollen and no anther cell was observed without forming the tapetum and the middle layer;while the anther development of Hong Cai-tai's Ogura male-sterile line was blocked without anther at the stage of microspore mother cell & tetrads of microspores,observing the tapetum cell abnormal and tetrads of microspores being extruded.In the end the tapetum cell and tetrads of microspores disaggregated.

关 键 词：红菜薹 波里马胞质雄性不育 萝卜胞质雄性不育 花药败育 

分 类 号：Q942.4[生物学—植物学] S634.5[农业科学—蔬菜学]