利用农杆菌介导法将抗虫基因和高赖氨酸蛋白基因导入超级杂交水稻亲本材料1826中  

Introduction of Insect Resistance Gene and Lysine-rich Gene into Super Hybrid Rice Parental Line 1826 through Agrobacterium tumefaciens-mediated Trasnformation

作　　者：孔维文[1,2] 邓仲香[1] 王倩[1] 唐克轩[1] 

机构地区：[1]复旦大学生命科学学院遗传工程国家重点实验室,上海200433 [2]中国科学院遗传与发育生物学研究所

出　　处：《分子植物育种》2004年第4期495-500,共6页Molecular Plant Breeding

基　　金：国家十五863计划项目资助(项目编号:2001AA212091)

摘　　要：超级杂交水稻的培育、推广和应用可显著提高水稻的单位面积产量,但许多超级杂交水稻存在抗虫能力较低、品质较差的问题。1826是一个优良的超级杂交水稻亲本材料,但抗褐飞虱能力低,必需氨基酸赖氨酸的含量低。本研究利用基因工程技术将CaMV 35S启动子引导的半夏凝集素基因(PTA)和水稻胚乳特异表达启动子(Glutelin-B1 promoter)引导的马铃薯高赖氨酸蛋白基因(SB401)同时转入1826成熟胚诱导的愈伤组织中,获得了同时含PTA和SB401的转基因植株。本研究为提高1826的抗褐飞虱能力和赖氨酸含量打下了基础。Super rice breeding, spreading and application is an important measure to increase rice yield. However, many super rice varieties or combinations have poor insect resistance and grain quality lacking of essential amino acids such as lysine. 1826 is an elite super rice parental line but with poor resistance to Nilaparuata lugens (Stal) and with low lysine content. In the present study, PINELLIA TERNATA AGGLUTININ gene (PTA) under the control of Cauliflower mosaic virus 35S promoter and POTATO LYSINE-RICH gene (SB401) under the control of glutelin-B1 promoter were simultaneously introduced into 1826 mature embro seed-derived calli via Agrobacterium tumfaciens-mediated transformation in order to increase 1826's resistance to Nilaparuata lugens and lysine content. Transgenic rice plants contained PTA and SB401 genes were generated through antibiotic selection. This work provides a possibility to improve 1826's resistance to Nilaparuata lugens and lysine content.

关 键 词：农杆菌介导法 抗虫基因 高赖氨酸 蛋白基因导入 超级杂交水稻 亲本材料 1826 

分 类 号：S511[农业科学—作物学]