酶联免疫吸附和胶体金与荧光定量PCR技术在乙肝病毒检测中的应用  

Application of ELISA,gold colloid and FQ-PCR in detection of hepatitis B virus

作　　者：许丽艳[1] 敬华[1] 刘春雷[1] 周晓楠 陈兴明[1] 肖敏[1] 贾丽萍[1] 刘坤 魏平[1] 邴岩[1] 

机构地区：[1]解放军第306医院,北京市100101

出　　处：《总装备部医学学报》2007年第1期10-12,共3页Medical Journal of General Equipment Headquarters

摘　　要：目的探讨酶联免疫吸附(ELSIA)、胶体金与荧光定量PCR检测方法在乙肝病毒检测中的应用价值。方法采用ELSIA分析法及快速胶体金方法分别对标准品血清、阴阳性对照血清及43份随机抽样血清进行乙肝两对半检测;对乙肝两对半中不同项目阳性的68份血清标本做荧光定量PCR(FQ-PCR)检测,并将检测结果进行对比分析。结果ELSIA、胶体金两种方法检测结果比较,HBsAg、HBsAb阳性符合率达90.2%,HBeAg阳性符合率达到100%,HBeAb、HBcAb阳性符合率分别为80.1%、77.8%。HBsAg、HBeAg及HBcAb均阳性者荧光定量PCR阳性率达93.33%。结论ELSIA方法具有较好的灵敏度、特异性。胶体金检测方法可以避免Hock效应所致的假阴性结果,可作为急诊筛查检测方法。HBeAg阳性与HBVDNA阳性吻合度最高,是判定病毒复制与传染性的可靠指标。Objective To investigate the value of ELSIA,gold colloid and FQ-PCR in the diagnosis of hepatitis B virus.Methods Hepatitis B viruses were detected by ELISA,gold colloid in the standard serum,negative and positive serum and 43 random samples;hepatitis B viruses were also detected by FQ-PCR in 63 positive serum samples;The results were compared with each other.Results HbsAg and HBsAb positive accordant rates were 90.2% as compared ELISA with gold colloid methods.HBeAg positive accordant rate were 100%;HbeAb and HBcAb positive accordant rates were 80.1% and 77.8% respectively.PCR positive rate was 93.33% in samples of all the HBsAg,HBeAg and HBcAb positive cases.Conclusions ELISA has fine sensitivity and specificity.Gold colloid can avoid false negative results in Hock's effect,and it can be a screen method.HBeAg positivity has higher accordance with HBVDNA positivity,which is a credible indicator in determination of virus copy and infection.

关 键 词：乙肝两对半 酶联免疫吸附 PCR 

分 类 号：R450[医药卫生—治疗学]