反相高效液相色谱法同时测定复方氯霉素醇溶液中氯霉素与水杨酸的含量  

Simultaneous determination of chloramphenicol and salicylic acid contents in compound chloramphenicol alcoholic solution by reverse phase high-performance liquid chromatography-HPLC

作　　者：夏鹏霄 杨瀚春 杨粉霞 崔磊 

机构地区：[1]解放军513医院药剂科,甘肃兰州732750

出　　处：《总装备部医学学报》2014年第1期22-24,共3页Medical Journal of General Equipment Headquarters

摘　　要：目的建立复方氯霉素醇溶液中氯霉素和水杨酸含量的测定方法。方法采用反相高效液相色谱法(RP-HPLC),色谱柱:Shim-pack VP-ODS C18柱(4.6mm×250mm,5μm);流动相:甲醇-1%乙酸(50∶50);流速:1.0ml/min;检测波长:293nm。结果氯霉素在0.02506~0.4010mg/ml浓度范围内呈良好线性关系,r=0.9998,平均回收率99.8%,相对标准偏差(relative standard deviation,RSD)为1.2%。水杨酸在0.02520~0.4032 mg/ml浓度范围内呈良好线性关系,r=0.9997,平均回收率99.9%,RSD为1.6%。结论本方法简便、准确,可用于同时测定复方氯霉素醇溶液中氯霉素和水杨酸的含量。Objective To establish a method for the simultaneous determination of chloramphenicol and salicylic acid in compound chloramphenicol alcoholic solution. Methods The content of salicylic acid and chloramphenicol was determined by reverse phase high-performance liquid chromatography(RP-HPLC)on a Shim-pack VP-ODS C18 column(250mm×4.6mm,5μm).The mobile phase consisted of methanol-1% acetic acid solution(50∶50).The flow rate was 1.0ml/ min,and the detection wavelength was 293nm. Results Chloramphenicol showed a good linear relationship within a range of 0.02506~0.4010mg/ml,r=0.9998;the average recovery was 99.8% and RSD was 1.2%.The linear range of salicylic acid was 0.02520~0.4032mg/ml,r=0.9997,and the average recovery was 99.9%,with an RSD of 1.6%. Conclusions This method is simple and accurate,and suitable for determining chloramphenicol and salicylic acid in compound chloramphenicol alcoholic solution.

关 键 词：氯霉素 水杨酸 复方氯霉素醇溶液 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]