蛇毒F_(6.3.2)激活蛋白C的可能机制初探  

The Possible Activating Mechanism of F6.3.2 of Snake Venom on Protein C

作　　者：侯力强[1] 赵路宁[1] 管锦霞[1] 

机构地区：[1]广州医学院广州蛇毒研究所,广州510182

出　　处：《中国医学生物技术应用》2004年第2期12-16,共5页The Chinese Academic Medical Magazine of Organisms

基　　金：广东省医学科学技术研究基金资助项目(B2000061);广州市教委科研项目基金

摘　　要：本实验研究蛇毒蛋白C激活组份F6.3.2对蛋白C的激活作用。以BaCl2吸附血浆和纯化蛋白C为作用底物,采用发色底物法测定蛇毒组份F6.3.2对蛋白C的特异性激活作用,同时采用SDS-PAGE.测定该组份对蛋白C的激活机制。结果发现F6.3.2没有直接作用发色底物使之生色的能力。它对BaCl2吸附血浆不表现生色反应能力(与对照组相比P>0.05),而对纯化蛋白C则表现出很强的生色反应能力(与对照组Ⅰ、对照组Ⅱ相比P<0.01).SDS-PAGE结果表明,F6.3.2与纯化蛋白C作用后的混合物,由两条链组成,一条链分子量为19KDa,与纯化蛋白C的轻链相同,另一条链分子量为59.5 KDa。为纯化蛋白C重链与F6.3.2的分子量之和,表明F6.3.2已结合到纯化蛋白C的重链上。因此.我们认为蛇毒蛋白C激活组份F6.3.2对蛋白C有特异性激活作用,它激活蛋白C的可能机制是通过结合于蛋白C的重链,形成F6.3.2-蛋白C复合物,引起蛋白C变构,从而将蛋白C激活为活化蛋白C。The purpose of this paper was to investigate the activating effect of a Protein-C-activating Fraction F6.3.2 from the snake venom on protein C. Using barium chloride absorption plasma and purified protein C as reaction substrates, chromogenic substrate method was employed to determine the specific activating effect of F6.3.2 on protein C; SDS-PAGE was employed to investigate the protein-C-activating mechanism of F6.3.2. F6.3.2,which has no directly chromogenic ability towards chromogenic substrate, exhibits apparent chromogenic ability to purified protein C(P<0.01). SDS-PAGE shows that F6.3.2 mixture with purified protein C consists of two single-chain polypeptides with molecule weights of 19KDa and 59.5 KDa. It indicates that F6.3.2 has connected with the heavy chain of protein C. We concluded that F6.3.2 has specific activating effect on protein C. F6.3.2 was firstly connected with the heavy chain of protein C, formed F6.3.2 complex with protein C,and then caused activation.

关 键 词：蛇毒 蛋白C 

分 类 号：R284[医药卫生—中药学]