Luminex液相芯片技术检测与胰岛素抵抗相关的脂肪细胞因子水平在妊娠期糖耐量受损孕妇中的相关性研究  

Association of cytokines level with insulin resistance in pregnancy women with gestational impaired glucose tolerance

作　　者：钱源[1] 宗迪[2] 马润玫[1] 褚嘉佑[2] 孙浩[2] 

机构地区：[1]昆明医科大学第一附属医院妇产科,云南昆明650032 [2]中国医学科学院医学生物学研究所,云南昆明650118

出　　处：《中国产前诊断杂志（电子版）》2012年第4期10-13,共4页Chinese Journal of Prenatal Diagnosis(Electronic Version)

基　　金：昆明医科大学第一附属医院院内资助项目"引起胰岛素抵抗的脂肪细胞分泌因子与妊娠期糖尿病易感性的研究"

摘　　要：目的研究汉族妇女血清中的与胰岛素抵抗相关脂肪细胞因子水平,并且比较在妊娠期正常血糖水平组和糖耐量受损组中的水平差异。方法采用Luminex液相芯片技术对IL-6、TNF-α、瘦素、MCP-1等脂肪细胞因子在共80例孕妇血清中的表达水平进行检测,并进行差异性检测。结果瘦素水平在2组中具有显著性差异(P<0.01),其他各细胞因子则无明显区别(P>0.05)。结论血清瘦素水平与妊娠期糖耐量受损(GIGT)密切相关,如果改善血清瘦素水平,提高胰岛素敏感性,将对GIGT的防治有重要的临床意义。而IL-6、TNF-α、MCP-1等细胞因子水平与GIGT则无关,其与GIGT的关系需要进一步的研究。Objective To investigate the level of cytokines associated with insulin resistance in serum of Chinese Han pregnancy women and to compare the discrepancy of the cytokine level between the control group and GIGT group. Method 40 samples in each of the impaired glucose tolerance group and the control group were selected, using Luminex multiplex analysis for the detection of cytokines level. The discrepancy between the two groups was detected using χ2 analysis. Results The results showed that the serum Leptin level has significant difference between GIGT group and control group (P<0.05), and interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), monocyte chemo attractant protein 1 (MCP-1) have no obvious difference between the two groups (P>0.05). Conclusions The result support that the level of Leptin was closely associated with the pathogenesis of the GIGT, which makes Leptin as a potential marker for gestational impaired glucose tolerance. The high level of expression of Leptin causes insulin resistance, while the latter is one of the major reason for abnormal glucose tolerance. There was no association between the level of IL-6, TNF-αand MCP-1 and pathogenesis of the GIGT. Futher studies are needed to detect the relationship between them.

关 键 词：液相芯片 瘦素 胰岛素抵抗 糖耐量受损 

分 类 号：R587.1[医药卫生—内分泌]